2CDX

STRUCTURE OF COBRA CARDIOTOXIN CTXI AS DERIVED FROM NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY AND DISTANCE GEOMETRY CALCULATIONS

2CDX の概要

• エントリーDOI: 10.2210/pdb2cdx/pdb
• 分子名称: CARDIOTOXIN CTX I (1 entity in total)
• 機能のキーワード: cardiotoxin
• 由来する生物種: Naja atra (Chinese cobra)
• 細胞内の位置: Secreted : P60304
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6711.23

構造登録者

Jahnke, W.,Mierke, D.F.,Beress, L.,Kessler, H. (登録日: 1994-09-06, 公開日: 1994-11-30, 最終更新日: 2024-11-20)

主引用文献

Jahnke, W.,Mierke, D.F.,Beress, L.,Kessler, H.
Structure of cobra cardiotoxin CTX I as derived from nuclear magnetic resonance spectroscopy and distance geometry calculations.
J.Mol.Biol., 240:445-458, 1994

PubMed Abstract: The structure of cardiotoxin CTX I from Naja naja atra has been investigated by NMR spectroscopy. Sequence specific resonance assignments have been obtained for all backbone protons as well as for most side-chain protons. Distance geometry calculations were carried out using a metric matrix DG program. A total of 715 NOE constraints, 27 phi angle constraints and a list of the hydrogen bond donors were used for the metric matrix DG calculations and refinement. The average pairwise r.m.s.d. of the resulting structures was 1.01 A for the backbone heavy atoms, and 1.69 A for all heavy atoms. The protein is rich in beta structure and consists of a large triple-stranded, antiparallel beta sheet as well as a short double-stranded, antiparallel beta sheet. Non-regular hydrogen bonding is found between side-chains of the carboxy-terminal end and the rest of the core region. The structure is discussed in terms of evolutionary aspects as well as recent investigations about the biological function and active site.

PubMed: 8046750
DOI: 10.1006/jmbi.1994.1460

実験手法

SOLUTION NMR