2CAY

Vps36 N-terminal PH domain

2CAY の概要

• エントリーDOI: 10.2210/pdb2cay/pdb
• 関連するPDBエントリー: 1U5T 1W7P
• 分子名称: VACUOLAR PROTEIN SORTING PROTEIN 36, SULFATE ION (3 entities in total)
• 機能のキーワード: transport, vps36, ph domain, escrt-ii, lipid-binding, multivesicular bodies, membrane, metal-binding, protein transport, zinc, zinc-finger
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• 細胞内の位置: Cytoplasm : Q06696
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33978.16

構造登録者

Teo, H.,Williams, R.L.,Perisic, O.,Gill, D.J. (登録日: 2005-12-23, 公開日: 2006-04-07, 最終更新日: 2024-05-08)

主引用文献

Teo, H.,Gill, D.J.,Sun, J.,Perisic, O.,Veprintsev, D.B.,Wallis, Y.,Emr, S.D.,Williams, R.L.
Escrt-I Core and Escrt-II Glue Domain Structures Reveal Role for Glue in Linking to Escrt-I and Membranes.
Cell(Cambridge,Mass.), 125:99-, 2006

PubMed Abstract: ESCRT complexes form the main machinery driving protein sorting from endosomes to lysosomes. Currently, the picture regarding assembly of ESCRTs on endosomes is incomplete. The structure of the conserved heterotrimeric ESCRT-I core presented here shows a fan-like arrangement of three helical hairpins, each corresponding to a different subunit. Vps23/Tsg101 is the central hairpin sandwiched between the other subunits, explaining the critical role of its "steadiness box" in the stability of ESCRT-I. We show that yeast ESCRT-I links directly to ESCRT-II, through a tight interaction of Vps28 (ESCRT-I) with the yeast-specific zinc-finger insertion within the GLUE domain of Vps36 (ESCRT-II). The crystal structure of the GLUE domain missing this insertion reveals it is a split PH domain, with a noncanonical lipid binding pocket that binds PtdIns3P. The simultaneous and reinforcing interactions of ESCRT-II GLUE domain with membranes, ESCRT-I, and ubiquitin are critical for ubiquitinated cargo progression from early to late endosomes.

PubMed: 16615893
DOI: 10.1016/J.CELL.2006.01.047

実験手法

X-RAY DIFFRACTION (1.9 Å)