2CA5
MxiH needle protein of Shigella Flexneri (monomeric form, residues 1- 78)
Summary for 2CA5
| Entry DOI | 10.2210/pdb2ca5/pdb |
| Descriptor | MXIH, ISOPROPYL ALCOHOL, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | transport protein, mxih, type iii secretion system, needle complex, protein transport, virulence |
| Biological source | SHIGELLA FLEXNERI |
| Total number of polymer chains | 2 |
| Total formula weight | 19349.29 |
| Authors | Deane, J.E.,Roversi, P.,Cordes, F.S.,Johnson, S.,Kenjale, R.,Picking, W.L.,Picking, W.D.,Blocker, A.J.,Lea, S.M. (deposition date: 2005-12-16, release date: 2006-08-07, Last modification date: 2024-05-08) |
| Primary citation | Deane, J.E.,Roversi, P.,Cordes, F.S.,Johnson, S.,Kenjale, R.,Daniell, S.,Booy, F.,Picking, W.L.,Picking, W.D.,Blocker, A.J.,Lea, S.M. Molecular Model of a Type III Secretion System Needle: Implications for Host-Cell Sensing Proc.Natl.Acad.Sci.USA, 103:12529-, 2006 Cited by PubMed Abstract: Type III secretion systems are essential virulence determinants for many Gram-negative bacterial pathogens. The type III secretion system consists of cytoplasmic, transmembrane, and extracellular domains. The extracellular domain is a hollow needle protruding above the bacterial surface and is held within a basal body that traverses both bacterial membranes. Effector proteins are translocated, via this external needle, directly into host cells, where they subvert normal cell functions to aid infection. Physical contact with host cells initiates secretion and leads to formation of a pore, thought to be contiguous with the needle channel, in the host-cell membrane. Here, we report the crystal structure of the Shigella flexneri needle subunit MxiH and a complete model for the needle assembly built into our three-dimensional EM reconstruction. The model, combined with mutagenesis data, reveals that signaling of host-cell contact is relayed through the needle via intersubunit contacts and suggests a mode of binding for a tip complex. PubMed: 16888041DOI: 10.1073/PNAS.0602689103 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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