2C79

The structure of a family 4 acetyl xylan esterase from Clostridium thermocellum in complex with a colbalt ion.

2C79 の概要

• エントリーDOI: 10.2210/pdb2c79/pdb
• 関連するPDBエントリー: 2C71
• 分子名称: GLYCOSIDE HYDROLASE, FAMILY 11\:CLOSTRIDIUM CELLULOSOME ENZYME, DOCKERIN TYPE I\:POLYSACCHARIDE, COBALT (II) ION (3 entities in total)
• 機能のキーワード: acetyl-xylan, esterases, metal-ion, hydrolase
• 由来する生物種: CLOSTRIDIUM THERMOCELLUM
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23989.15

構造登録者

Taylor, E.J.,Turkenburg, P.J.,Vincent, F.,Brzozowski, A.M.,Gloster, T.M.,Dupont, C.,Shareck, F.,Centeno, M.S.J.,Prates, J.A.M.,Ferreira, L.M.A.,Fontes, C.M.G.A.,Biely, P.,Davies, G.J. (登録日: 2005-11-18, 公開日: 2006-01-23, 最終更新日: 2023-12-13)

主引用文献

Taylor, E.J.,Gloster, T.M.,Turkenburg, P.J.,Vincent, F.,Brzozowski, A.M.,Dupont, C.,Shareck, F.,Centeno, M.S.J.,Prates, J.A.M.,Puchart, V.,Ferreira, L.M.A.,Fontes, C.M.G.A.,Biely, P.,Davies, G.J.
Structure and Activity of Two Metal-Ion Dependent Acetyl Xylan Esterases Involved in Plant Cell Wall Degradation Reveals a Close Similarity to Peptidoglycan Deacetylases.
J.Biol.Chem., 281:10968-, 2006

PubMed Abstract: The enzymatic degradation of plant cell wall xylan requires the concerted action of a diverse enzymatic syndicate. Among these enzymes are xylan esterases, which hydrolyze the O-acetyl substituents, primarily at the O-2 position of the xylan backbone. All acetylxylan esterase structures described previously display a alpha/beta hydrolase fold with a "Ser-His-Asp" catalytic triad. Here we report the structures of two distinct acetylxylan esterases, those from Streptomyces lividans and Clostridium thermocellum, in native and complex forms, with x-ray data to between 1.6 and 1.0 A resolution. We show, using a novel linked assay system with PNP-2-O-acetylxyloside and a beta-xylosidase, that the enzymes are sugar-specific and metal ion-dependent and possess a single metal center with a chemical preference for Co2+. Asp and His side chains complete the catalytic machinery. Different metal ion preferences for the two enzymes may reflect the surprising diversity with which the metal ion coordinates residues and ligands in the active center environment of the S. lividans and C. thermocellum enzymes. These "CE4" esterases involved in plant cell wall degradation are shown to be closely related to the de-N-acetylases involved in chitin and peptidoglycan degradation (Blair, D. E., Schuettelkopf, A. W., MacRae, J. I., and Aalten, D. M. (2005) Proc. Natl. Acad. Sci. U. S. A., 102, 15429-15434), which form the NodB deacetylase "superfamily."

PubMed: 16431911
DOI: 10.1074/JBC.M513066200

実験手法

X-RAY DIFFRACTION (1.5 Å)