2C62

Crystal Structure of the Human Transcription Cofactor PC4 in Complex with Single-Stranded DNA

2C62 の概要

• エントリーDOI: 10.2210/pdb2c62/pdb
• 関連するPDBエントリー: 1PCF
• 分子名称: ACTIVATED RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR P15, 5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP *TP*TP*TP*TP*TP*TP*TP*TP*TP*G)-3', SULFATE ION, ... (4 entities in total)
• 機能のキーワード: transcription cofactor, single-stranded dna, protein-dna complex, dna unwinding, activator, dna-binding, nuclear protein, phosphorylation, transcription, transcription regulation
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus: P53999
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 21824.09

構造登録者

Werten, S.,Moras, D. (登録日: 2005-11-07, 公開日: 2006-01-11, 最終更新日: 2023-12-13)

主引用文献

Werten, S.,Moras, D.
A Global Transcription Cofactor Bound to Juxtaposed Strands of Unwound DNA
Nat.Struct.Mol.Biol., 13:181-, 2006

PubMed Abstract: The 1.74-A crystal structure of the human transcription cofactor PC4 in complex with a single-stranded 20-mer oligonucleotide reveals how symmetry-related beta-surfaces of the protein homodimer interact with juxtaposed 5-nucleotide DNA regions running in opposite directions. The structure explains high-affinity binding of PC4 to the complementary strands of unwinding duplex DNA, and it suggests the cofactor may have a role in relaxing negative supercoils or exposing unpaired bases for sequence-specific recognition by other biomolecules.

PubMed: 16415882
DOI: 10.1038/NSMB1044

実験手法

X-RAY DIFFRACTION (1.74 Å)