2C61

Crystal structure of the non-catalytic B subunit of A-type ATPase from M. mazei Go1

2C61 の概要

• エントリーDOI: 10.2210/pdb2c61/pdb
• 分子名称: A-TYPE ATP SYNTHASE NON-CATALYTIC SUBUNIT B (2 entities in total)
• 機能のキーワード: hydrolase, a-type atp synthase, h+ atpase, a1ao, non-catalytic, atp synthesis, hydrogen ion transport, ion transport, transport
• 由来する生物種: METHANOSARCINA MAZEI GO1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 103109.34

構造登録者

Schaefer, I.,Bailer, S.M.,Dueser, M.,Boersch, M.,Bernal, R.A.,Grueber, G.,Stock, D. (登録日: 2005-11-06, 公開日: 2006-12-11, 最終更新日: 2024-05-08)

主引用文献

Schaefer, I.,Bailer, S.M.,Dueser, M.,Boersch, M.,Bernal, R.A.,Stock, D.,Grueber, G.
Crystal Structure of the Archaeal A1Ao ATP Synthase Subunit B from Methanosarcina Mazei Go1: Implications of Nucleotide-Binding Differences in the Major A1Ao Subunits a and B. Subunits a and B
J.Mol.Biol., 358:725-, 2006

PubMed Abstract: The A1Ao ATP synthase from archaea represents a class of chimeric ATPases/synthases, whose function and general structural design share characteristics both with vacuolar V1Vo ATPases and with F1Fo ATP synthases. The primary sequences of the two large polypeptides A and B, from the catalytic part, are closely related to the eukaryotic V1Vo ATPases. The chimeric nature of the A1Ao ATP synthase from the archaeon Methanosarcina mazei Gö1 was investigated in terms of nucleotide interaction. Here, we demonstrate the ability of the overexpressed A and B subunits to bind ADP and ATP by photoaffinity labeling. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to map the peptide of subunit B involved in nucleotide interaction. Nucleotide affinities in both subunits were determined by fluorescence correlation spectroscopy, indicating a weaker binding of nucleotide analogues to subunit B than to A. In addition, the nucleotide-free crystal structure of subunit B is presented at 1.5 A resolution, providing the first view of the so-called non-catalytic subunit of the A1Ao ATP synthase. Superposition of the A-ATP synthase non-catalytic B subunit and the F-ATP synthase non-catalytic alpha subunit provides new insights into the similarities and differences of these nucleotide-binding ATPase subunits in particular, and into nucleotide binding in general. The arrangement of subunit B within the intact A1Ao ATP synthase is presented.

PubMed: 16563431
DOI: 10.1016/J.JMB.2006.02.057

実験手法

X-RAY DIFFRACTION (1.5 Å)