2C38

RNase PH core of the archaeal exosome in complex with A5 RNA

2C38 の概要

• エントリーDOI: 10.2210/pdb2c38/pdb
• 関連するPDBエントリー: 2BR2 2C37 2C39
• 分子名称: PROBABLE EXOSOME COMPLEX EXONUCLEASE 2, PROBABLE EXOSOME COMPLEX EXONUCLEASE 1, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: exosome, rnase ph, rrp41, rrp42, phosphorolytic, exoribonuclease, rna degradation, archaeal, hydrolase, exonuclease, nuclease
• 由来する生物種: SULFOLOBUS SOLFATARICUS; 詳細
• 細胞内の位置: Cytoplasm (Potential): Q9UXC0 Q9UXC2
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 704234.36

構造登録者

Lorentzen, E.,Conti, E. (登録日: 2005-10-04, 公開日: 2005-11-23, 最終更新日: 2024-11-20)

主引用文献

Lorentzen, E.,Conti, E.
Structural Basis of 3' End RNA Recognition and Exoribonucleolytic Cleavage by an Exosome Rnase Ph Core.
Mol.Cell, 20:473-, 2005

PubMed Abstract: The exosome is a macromolecular complex that plays fundamental roles in the biogenesis and turnover of a large number of RNA species. Here we report the crystal structures of the Rrp41-Rrp42 core complex of the S. solfataricus exosome bound to short single-stranded RNAs and to ADP. The RNA binding cleft recognizes four nucleotides in a sequence-unspecific manner, mainly by electrostatic interactions with the phosphate groups. Interactions at the 2' hydroxyls of the sugars provide specificity for RNA over DNA. The structures show both the bound substrate and the cleaved product of the reaction, suggesting a catalytic mechanism for the 3'-5' phosphorolytic activity of the exosome.

PubMed: 16285928
DOI: 10.1016/J.MOLCEL.2005.10.020

実験手法

X-RAY DIFFRACTION (3.1 Å)