2C1W

The structure of XendoU: a splicing independent snoRNA processing endoribonuclease

2C1W の概要

• エントリーDOI: 10.2210/pdb2c1w/pdb
• 関連するPDBエントリー: 2C09
• 分子名称: ENDOU PROTEIN, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: nuclease, snorna, endoribonuclease, splicing independent processing
• 由来する生物種: XENOPUS LAEVIS (AFRICAN CLAWED FROG)
• 細胞内の位置: Nucleus: Q8JFY9
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 102000.58

構造登録者

Renzi, F.,Caffarelli, E.,Laneve, P.,Bozzoni, I.,Brunori, M.,Vallone, B. (登録日: 2005-09-21, 公開日: 2006-07-20, 最終更新日: 2024-05-08)

主引用文献

Renzi, F.,Caffarelli, E.,Laneve, P.,Bozzoni, I.,Brunori, M.,Vallone, B.
The Structure of the Endoribonuclease Xendou: From Small Nucleolar RNA Processing to Severe Acute Respiratory Syndrome Coronavirus Replication.
Proc.Natl.Acad.Sci.USA, 103:12365-, 2006

PubMed Abstract: Small nucleolar RNAs (snoRNAs) play a key role in eukaryotic ribosome biogenesis. In most cases, snoRNAs are encoded in introns and are released through the splicing reaction. Some snoRNAs are, instead, produced by an alternative pathway consisting of endonucleolytic processing of pre-mRNA. XendoU, the endoribonuclease responsible for this activity, is a U-specific, metal-dependent enzyme that releases products with 2'-3' cyclic phosphate termini. XendoU is broadly conserved among eukaryotes, and it is a genetic marker of nidoviruses, including the severe acute respiratory syndrome coronavirus, where it is essential for replication and transcription. We have determined by crystallography the structure of XendoU that, by refined search methodologies, appears to display a unique fold. Based on sequence conservation, mutagenesis, and docking simulations, we have identified the active site. The conserved structural determinants of this site may provide a framework for attempting to design antiviral drugs to interfere with the infectious nidovirus life cycle.

PubMed: 16895992
DOI: 10.1073/PNAS.0602426103

実験手法

X-RAY DIFFRACTION (2.2 Å)