2C0N

Crystal Structure of A197 from STIV

2C0N の概要

• エントリーDOI: 10.2210/pdb2c0n/pdb
• 分子名称: A197, 1,4-DIETHYLENE DIOXIDE, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: viral protein-transferase complex, viral protein, virus, archaea, crenarchaea, archaeal virus, crenarchaeal virus, thermophilic protein, thermophilic virus, stiv, sulfolobus turreted icosahedral virus, sulfolobus, glycosyltransferase, transferase, viral protein/transferase
• 由来する生物種: SULFOLOBUS TURRETED ICOSAHEDRAL VIRUS (STIV)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24648.38

構造登録者

Larson, E.T.,Reiter, D.,Young, M.,Lawrence, C.M. (登録日: 2005-09-06, 公開日: 2005-09-29, 最終更新日: 2024-10-16)

主引用文献

Larson, E.T.,Reiter, D.,Young, M.,Lawrence, C.M.
Structure of A197 from Sulfolobus Turreted Icosahedral Virus: A Crenarchaeal Viral Glycosyltransferase Exhibiting the Gt-A Fold.
J.Virol., 80:7636-, 2006

PubMed Abstract: Sulfolobus turreted icosahedral virus (STIV) was the first icosahedral virus characterized from an archaeal host. It infects Sulfolobus species that thrive in the acidic hot springs (pH 2.9 to 3.9 and 72 to 92 degrees C) of Yellowstone National Park. The overall capsid architecture and the structure of its major capsid protein are very similar to those of the bacteriophage PRD1 and eukaryotic viruses Paramecium bursaria Chlorella virus 1 and adenovirus, suggesting a viral lineage that predates the three domains of life. The 17,663-base-pair, circular, double-stranded DNA genome contains 36 potential open reading frames, whose sequences generally show little similarity to other genes in the sequence databases. However, functional and evolutionary information may be suggested by a protein's three-dimensional structure. To this end, we have undertaken structural studies of the STIV proteome. Here we report our work on A197, the product of an STIV open reading frame. The structure of A197 reveals a GT-A fold that is common to many members of the glycosyltransferase superfamily. A197 possesses a canonical DXD motif and a putative catalytic base that are hallmarks of this family of enzymes, strongly suggesting a glycosyltransferase activity for A197. Potential roles for the putative glycosyltransferase activity of A197 and their evolutionary implications are discussed.

PubMed: 16840342
DOI: 10.1128/JVI.00567-06

実験手法

X-RAY DIFFRACTION (1.86 Å)