2BY0

Is radiation damage dependent on the dose-rate used during macromolecular crystallography data collection

Summary for 2BY0

• Entry DOI: 10.2210/pdb2by0/pdb
• Related: 2BHU 2BHY 2BHZ 2BXY 2BXZ 2BY1 2BY2 2BY3 2BY5 2BY6 2BY7 2BY8 2BY9 2BYA
• Related PRD ID: PRD_900006
• Descriptor: MALTOOLIGOSYLTREHALOSE TREHALOHYDROLASE, alpha-D-glucopyranose-(1-1)-alpha-D-glucopyranose, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (7 entities in total)
• Functional Keywords: data collection, radiation damage, dose-rate, synchrotron radiation, hydrolase
• Biological source: DEINOCOCCUS RADIODURANS
• Total number of polymer chains: 1
• Total formula weight: 68713.10

Authors

Leiros, H.-K.S.,Timmins, J.,Ravelli, R.B.G.,McSweeney, S.M. (deposition date: 2005-07-28, release date: 2006-02-06, Last modification date: 2020-07-29)

Primary citation

Leiros, H.-K.S.,Timmins, J.,Ravelli, R.B.G.,Mcsweeney, S.M.
Is Radiation Damage Dependent on the Dose-Rate Used During Macromolecular Crystallography Data Collection?
Acta Crystallogr.,Sect.D, 62:125-, 2006

PubMed Abstract: This paper focuses on the radiation-damage effects when applying the same total X-ray dose to protein crystals at different dose rates. These experiments have been performed on both a selenomethionated protein and on bovine trypsin using dose rates that span nearly two orders of magnitude. The results show no clear dose-rate effect on the global indicators of radiation damage, but a small measurable dose-rate effect could be found when studying specific radiation damage. It is hypothesized that this observed dose-rate effect relates to differences in the steady-state free-radical concentration.

PubMed: 16421442
DOI: 10.1107/S0907444905033627

Experimental method

X-RAY DIFFRACTION (1.55 Å)