2BTQ

Structure of BtubAB heterodimer from Prosthecobacter dejongeii

2BTQ の概要

• エントリーDOI: 10.2210/pdb2btq/pdb
• 関連するPDBエントリー: 2BTO
• 分子名称: TUBULIN BTUBA, TUBULIN BTUBB, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: structural protein, cytoskeletal protein-complex, bacterial tubulin, cytoskeleton, polymerization, verrucomicrobia, protein complex, cytoskeletal protein
• 由来する生物種: PROSTHECOBACTER DEJONGEII; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 98592.33

構造登録者

Schlieper, D.,Lowe, J. (登録日: 2005-06-06, 公開日: 2005-06-14, 最終更新日: 2023-12-13)

主引用文献

Schlieper, D.,Oliva, M.A.,Andreu, J.M.,Lowe, J.
Structure of Bacterial Tubulin Btuba/B: Evidence for Horizontal Gene Transfer.
Proc.Natl.Acad.Sci.USA, 102:9170-, 2005

PubMed Abstract: alphabeta-Tubulin heterodimers, from which the microtubules of the cytoskeleton are built, have a complex chaperone-dependent folding pathway. They are thought to be unique to eukaryotes, whereas the homologue FtsZ can be found in bacteria. The exceptions are BtubA and BtubB from Prosthecobacter, which have higher sequence homology to eukaryotic tubulin than to FtsZ. Here we show that some of their properties are different from tubulin, such as weak dimerization and chaperone-independent folding. However, their structure is strikingly similar to tubulin including surface loops, and BtubA/B form tubulin-like protofilaments. Presumably, BtubA/B were transferred from a eukaryotic cell by horizontal gene transfer because their high degree of similarity to eukaryotic genes is unique within the Prosthecobacter genome.

PubMed: 15967998
DOI: 10.1073/PNAS.0502859102

実験手法

X-RAY DIFFRACTION (3.2 Å)