2BT6

Ru(bpy)2(mbpy)-Modified Bovine Adrenodoxin

2BT6 の概要

• エントリーDOI: 10.2210/pdb2bt6/pdb
• 関連するPDBエントリー: 1AYF 1CJE 1E6E 1L6U 1L6V
• 分子名称: ADRENODOXIN 1, FE2/S2 (INORGANIC) CLUSTER, (4'-METHYL-2,2'BIPYRIDINE)BIS(2,2'-BIPYRIDINE), ... (5 entities in total)
• 機能のキーワード: ruthenium(ii) bipyridyl complex, intramolecular electron transfer, electron transport, metal-binding
• 由来する生物種: BOS TAURUS (BOVINE)
• 細胞内の位置: Mitochondrion matrix: P00257
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25268.44

構造登録者

Halavaty, A.,Mueller, J.J.,Contzen, J.,Jung, C.,Hannemann, F.,Bernhardt, R.,Galander, M.,Lendzian, F.,Heinemann, U. (登録日: 2005-05-26, 公開日: 2006-01-25, 最終更新日: 2024-11-06)

主引用文献

Halavaty, A.,Mueller, J.J.,Contzen, J.,Jung, C.,Hannemann, F.,Bernhardt, R.,Galander, M.,Lendzian, F.,Heinemann, U.
Light-Induced Reduction of Bovine Adrenodoxin Via the Covalently Bound Ruthenium(II) Bipyridyl Complex: Intramolecular Electron Transfer and Crystal Structure.
Biochemistry, 45:709-, 2006

PubMed Abstract: Bovine adrenodoxin (Adx) plays an important role in the electron-transfer process in the mitochondrial steroid hydroxylase system of the bovine adrenal cortex. Using electron paramagnetic resonance (EPR) spectroscopy, we showed that photoreduction of the [2Fe-2S] cluster of Adx via (4'-methyl-2,2'-bipyridine)bis(2,2'-bipyridine)ruthenium(II) [Ru(bpy)2(mbpy)] covalently attached to the protein surface can be used as a new approach to probe the "shuttle" hypothesis for the electron transfer by Adx. The 1.5 A resolution crystal structure of a 1:1 Ru(bpy)2(mbpy)-Adx(1-108) complex reveals the site of modification, Cys95, and allows to predict the possible intramolecular electron-transfer pathways within the complex. Photoreduction of uncoupled Adx, mutant Adx(1-108), and Ru(bpy)2(mbpy)-Adx(1-108) using safranin T as the mediating electron donor suggests that two electrons are transferred from the dye to Adx. The intramolecular photoreduction rate constant for the ruthenated Adx has been determined and is discussed according to the predicted pathways.

PubMed: 16411746
DOI: 10.1021/BI0510330

実験手法

X-RAY DIFFRACTION (1.5 Å)