2BSL

Crystal structure of L. lactis dihydroorotate dehydrogense A in complex with 3,4-dihydroxybenzoate

2BSL の概要

• エントリーDOI: 10.2210/pdb2bsl/pdb
• 関連するPDBエントリー: 1DOR 1JQV 1JQX 1JRB 1JRC 1JUB 1JUE 1NFC 1OVD 2DOR
• 分子名称: DIHYDROOROTATE DEHYDROGENASE A, FLAVIN MONONUCLEOTIDE, ACETATE ION, ... (7 entities in total)
• 機能のキーワード: nucleotide metabolism, dihydroorotate dehydrogenase, fmn, flavoprotein, oxidoreductase, pyrimidine biosynthesis
• 由来する生物種: LACTOCOCCUS LACTIS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69750.89

構造登録者

Wolfe, A.E.,Hansen, M.,Gattis, S.G.,Hu, Y.-C.,Johansson, E.,Arent, S.,Larsen, S.,Palfey, B.A. (登録日: 2005-05-23, 公開日: 2006-08-29, 最終更新日: 2023-12-13)

主引用文献

Wolfe, A.E.,Thymark, M.,Gattis, S.G.,Fagan, R.L.,Hu, Y.-C.,Johansson, E.,Arent, S.,Larsen, S.,Palfey, B.A.
Interaction of Benzoate Pyrimidine Analogues with Class 1A Dihydroorotate Dehydrogenase from Lactococcus Lactis.
Biochemistry, 46:5741-, 2007

PubMed Abstract: Dihydroorotate dehydrogenases (DHODs) catalyze the oxidation of dihydroorotate to orotate in the only redox reaction in pyrimidine biosynthesis. The pyrimidine binding sites are very similar in all structurally characterized DHODs, suggesting that the prospects for identifying a class-specific inhibitor directed against this site are poor. Nonetheless, two compounds that bind specifically to the Class 1A DHOD from Lactococcus lactis, 3,4-dihydroxybenzoate (3,4-diOHB) and 3,5-dihydroxybenzoate (3,5-diOHB), have been identified [Palfey et al. (2001) J. Med. Chem. 44, 2861-2864]. The mechanism of inhibitor binding to the Class 1A DHOD from L. lactis has now been studied in detail and is reported here. Titrations showed that 3,4-diOHB binds more tightly at higher pH, whereas the opposite is true for 3,5-diOHB. Isothermal titration calorimetry and absorbance spectroscopy showed that 3,4-diOHB ionizes to the phenolate upon binding to the enzyme, but 3,5-diOHB does not. The charge-transfer band that forms in the 3,4-diOHB complex allowed the kinetics of binding to be observed in stopped-flow experiments. Binding was slow enough to observe from pH 6 to pH 8 and was (minimally) a two-step process consisting of the rapid formation of a complex that isomerized to the final charge-transfer complex. Orotate and 3,5-diOHB bind too quickly to follow directly, but their dissociation kinetics were studied by competition and described adequately with a single step. Crystal structures of both inhibitor complexes were determined, showing that 3,5-diOHB binds in the same orientation as orotate. In contrast, 3,4-diOHB binds in a twisted orientation, enabling one of its phenolic oxygens to form a very strong hydrogen bond to an asparagine, thus stabilizing the phenolate and causing charge-transfer interactions with the pi-system of the flavin, resulting in a green color.

PubMed: 17444658
DOI: 10.1021/BI7001554

実験手法

X-RAY DIFFRACTION (2.3 Å)