2BRS

EMBP Heparin complex

2BRS の概要

• エントリーDOI: 10.2210/pdb2brs/pdb
• 関連するPDBエントリー: 1H8U
• 分子名称: EOSINOPHIL-GRANULE MAJOR BASIC PROTEIN, 2-O-sulfo-beta-L-altropyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: antibiotic, embp, eosinophil, eosinophil granule protein, glycoprotein, heparin-binding, immune response, lectin, proteoglycan, heparin
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Bone marrow proteoglycan: Secreted. Eosinophil granule major basic protein: Cytoplasmic vesicle, secretory vesicle: P13727
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29005.79

構造登録者

Swaminathan, G.J.,Myszka, D.G.,Katsamba, P.S.,Ohnuki, L.E.,Gleich, G.J.,Acharya, K.R. (登録日: 2005-05-11, 公開日: 2005-10-26, 最終更新日: 2024-10-16)

主引用文献

Swaminathan, G.J.,Myszka, D.G.,Katsamba, P.S.,Ohnuki, L.E.,Gleich, G.J.,Acharya, K.R.
Eosinophil-Granule Major Basic Protein, a C-Type Lectin, Binds Heparin
Biochemistry, 44:14152-, 2005

PubMed Abstract: The eosinophil major basic protein (EMBP), a constituent of the eosinophil secondary granule, is implicated in cytotoxicity and mediation of allergic disorders such as asthma. It is a member of the C-type lectin family, but lacks a Ca(2+)- and carbohydrate-binding site as seen in other members of this family. Here, we report the crystal structure of EMBP in complex with a heparin disaccharide and in the absence of Ca(2+), the first such report of any C-lectin with this sugar. We also provide direct evidence of binding of EMBP to heparin and heparin disaccharide by surface plasmon resonance. We propose that the sugars recognized by EMBP are likely to be proteoglycans such as heparin, leading to new interpretations for EMBP function.

PubMed: 16245931
DOI: 10.1021/BI051112B

実験手法

X-RAY DIFFRACTION (2.2 Å)