2BPS

Ubiquitin-like protein YukD of Bacillus subtilis

Summary for 2BPS

• Entry DOI: 10.2210/pdb2bps/pdb
• Descriptor: YUKD PROTEIN (2 entities in total)
• Functional Keywords: yukd, ubiquitin-like protein, ubiquitin
• Biological source: BACILLUS SUBTILIS
• Total number of polymer chains: 2
• Total formula weight: 18481.04

Authors

van den Ent, F.,Lowe, J. (deposition date: 2005-04-22, release date: 2005-07-04, Last modification date: 2024-05-08)

Primary citation

van den Ent, F.,Lowe, J.
Crystal Structure of Ubiquitin-Like Protein Yukd from Bacillus Subtilis
FEBS Lett., 579:3837-, 2005

PubMed Abstract: The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. The endogenous gene of yukD in B. subtilis was disrupted without an obvious phenotypic effect and an inducible copy encoding a C-Myc and His-tagged version of the protein was introduced at the ectopic locus amyE. Conjugation assays performed both in vitro and in vivo indicate that YukD lacks the capacity for covalent bond formation with other proteins.

PubMed: 15978580
DOI: 10.1016/J.FEBSLET.2005.06.002

Experimental method

X-RAY DIFFRACTION (2.7 Å)