2BPS

Ubiquitin-like protein YukD of Bacillus subtilis

2BPS の概要

• エントリーDOI: 10.2210/pdb2bps/pdb
• 分子名称: YUKD PROTEIN (2 entities in total)
• 機能のキーワード: yukd, ubiquitin-like protein, ubiquitin
• 由来する生物種: BACILLUS SUBTILIS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18481.04

構造登録者

van den Ent, F.,Lowe, J. (登録日: 2005-04-22, 公開日: 2005-07-04, 最終更新日: 2024-05-08)

主引用文献

van den Ent, F.,Lowe, J.
Crystal Structure of Ubiquitin-Like Protein Yukd from Bacillus Subtilis
FEBS Lett., 579:3837-, 2005

PubMed Abstract: The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. The endogenous gene of yukD in B. subtilis was disrupted without an obvious phenotypic effect and an inducible copy encoding a C-Myc and His-tagged version of the protein was introduced at the ectopic locus amyE. Conjugation assays performed both in vitro and in vivo indicate that YukD lacks the capacity for covalent bond formation with other proteins.

PubMed: 15978580
DOI: 10.1016/J.FEBSLET.2005.06.002

実験手法

X-RAY DIFFRACTION (2.7 Å)