2BPA

ATOMIC STRUCTURE OF SINGLE-STRANDED DNA BACTERIOPHAGE PHIX174 AND ITS FUNCTIONAL IMPLICATIONS

Summary for 2BPA

• Entry DOI: 10.2210/pdb2bpa/pdb
• Descriptor: DNA (5'-D(*AP*AP*AP*AP*C)-3'), PROTEIN (SUBUNIT OF BACTERIOPHAGE PHIX174), ... (5 entities in total)
• Functional Keywords: protein-dna complex, single strand, icosahedral virus, virus-dna complex, virus/dna
• Biological source: Enterobacteria phage phiX174; More
• Cellular location: Virion: P03641 P03643 P69592
• Total number of polymer chains: 4
• Total formula weight: 73089.90

Authors

McKenna, R.,Xia, D.,Willingmann, P.,Ilag, L.L.,Krishnaswamy, S.,Rossmann, M.G.,Olson, N.H.,Baker, T.S.,Incardona, N.L. (deposition date: 1991-12-03, release date: 1991-12-03, Last modification date: 2024-02-14)

Primary citation

McKenna, R.,Xia, D.,Willingmann, P.,Ilag, L.L.,Krishnaswamy, S.,Rossmann, M.G.,Olson, N.H.,Baker, T.S.,Incardona, N.L.
Atomic structure of single-stranded DNA bacteriophage phi X174 and its functional implications.
Nature, 355:137-143, 1992

PubMed Abstract: The mechanism of DNA ejection, viral assembly and evolution are related to the structure of bacteriophage phi X174. The F protein forms a T = 1 capsid whose major folding motif is the eight-stranded antiparallel beta barrel found in many other icosahedral viruses. Groups of 5 G proteins form 12 dominating spikes that enclose a hydrophilic channel containing some diffuse electron density. Each G protein is a tight beta barrel with its strands running radially outwards and with a topology similar to that of the F protein. The 12 'pilot' H proteins per virion may be partially located in the putative ion channel. The small, basic J protein is associated with the DNA and is situated in an interior cleft of the F protein. Tentatively, there are three regions of partially ordered DNA structure,

PubMed: 1370343
DOI: 10.1038/355137a0

Experimental method

X-RAY DIFFRACTION (3 Å)