2BO8

DISSECTION OF MANNOSYLGLYCERATE SYNTHASE: AN ARCHETYPAL MANNOSYLTRANSFERASE

2BO8 の概要

• エントリーDOI: 10.2210/pdb2bo8/pdb
• 関連するPDBエントリー: 2BO4 2BO6 2BO7
• 分子名称: MANNOSYLGLYCERATE SYNTHASE, GUANOSINE 5'-(TRIHYDROGEN DIPHOSPHATE), P'-D-MANNOPYRANOSYL ESTER, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: catalysis, glycosyltransferase, mannose, transferase, stereoselectivity
• 由来する生物種: RHODOTHERMUS MARINUS
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 468961.89

構造登録者

Flint, J.,Taylor, E.,Yang, M.,Bolam, D.N.,Tailford, L.E.,Martinez-Fleites, C.,Dodson, E.J.,Davis, B.G.,Gilbert, H.J.,Davies, G.J. (登録日: 2005-04-08, 公開日: 2005-06-06, 最終更新日: 2023-12-13)

主引用文献

Flint, J.,Taylor, E.,Yang, M.,Bolam, D.N.,Tailford, L.E.,Martinez-Fleites, C.,Dodson, E.J.,Davis, B.G.,Gilbert, H.J.,Davies, G.J.
Structural dissection and high-throughput screening of mannosylglycerate synthase.
Nat. Struct. Mol. Biol., 12:608-614, 2005

PubMed Abstract: The enzymatic transfer of activated mannose yields mannosides in glycoconjugates and oligo- and polysaccharides. Yet, despite its biological necessity, the mechanism by which glycosyltransferases recognize mannose and catalyze its transfer to acceptor molecules is poorly understood. Here, we report broad high-throughput screening and kinetic analyses of both natural and synthetic substrates of Rhodothermus marinus mannosylglycerate synthase (MGS), which catalyzes the formation of the stress protectant 2-O-alpha-D-mannosyl glycerate. The sequence of MGS indicates that it is at the cusp of inverting and retaining transferases. The structures of apo MGS and complexes with donor and acceptor molecules, including GDP-mannose, combined with mutagenesis of the binding and catalytic sites, unveil the mannosyl transfer center. Nucleotide specificity is as important in GDP-D-mannose recognition as the nature of the donor sugar.

PubMed: 15951819
DOI: 10.1038/nsmb950

実験手法

X-RAY DIFFRACTION (2.8 Å)