2BMJ

GTPase like domain of Centaurin Gamma 1 (Human)

2BMJ の概要

• エントリーDOI: 10.2210/pdb2bmj/pdb
• 分子名称: CENTAURIN GAMMA 1 (2 entities in total)
• 機能のキーワード: gtpase, structural genomics consortium, sgc, centaurin ank repeat, gtp-binding, hydrolase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Isoform 1: Cytoplasm. Isoform 2: Cytoplasm: Q99490
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19607.29

構造登録者

Yang, X.,Elkins, J.M.,Soundararajan, M.,Arrowsmith, C.,Edwards, A.,Sundstrom, M.,Doyle, D.A.,Structural Genomics Consortium (SGC) (登録日: 2005-03-14, 公開日: 2005-04-12, 最終更新日: 2023-12-13)

主引用文献

Soundararajan, M.,Yang, X.,Elkins, J.M.,Sobott, F.,Doyle, D.A.
The Centaurin Gamma-1 Gtpase-Like Domain Functions as an Ntpase.
Biochem.J., 401:679-, 2007

PubMed Abstract: Centaurins are a family of proteins that contain GTPase-activating protein domains, with the gamma family members containing in addition a GTPase-like domain. Centaurins reside mainly in the nucleus and are known to activate phosphoinositide 3-kinase, a key regulator of cell proliferation, motility and vesicular trafficking. In the present study, using X-ray structural analysis, enzymatic assays and nucleotide-binding studies, we show that, for CENTG1 (centaurin gamma-1) the GTPase-like domain has broader trinucleotide specificity. Alterations within the G4 motif of CENTG1 from the highly conserved NKXD found in typical GTPases to TQDR result in the loss of specificity, a lower affinity for the nucleotides and higher turnover rates. These results indicate that the centaurins could be more accurately classified as NTPases and point to alternative mechanisms of cell signalling control.

PubMed: 17037982
DOI: 10.1042/BJ20060555

実験手法

X-RAY DIFFRACTION (2.1 Å)