2BL6

Solution structure of the Zn complex of EIAV NCp11(22-58) peptide, including two CCHC Zn-binding motifs.

2BL6 の概要

• エントリーDOI: 10.2210/pdb2bl6/pdb
• 関連するPDBエントリー: 1EIA 1HEK 2EIA
• NMR情報: BMRB: 7352
• 分子名称: NUCLEOCAPSID PROTEIN P11, ZINC ION (2 entities in total)
• 機能のキーワード: nucleocapsid protein, lentivirus, polyprotein, core protein, retrovirus zinc finger-like domains
• 由来する生物種: EQUINE INFECTIOUS ANEMIA VIRUS (EIAV)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4283.69

構造登録者

Amodeo, P.,Castiglione-Morelli, M.A.,Ostuni, A.,Bavoso, A. (登録日: 2005-03-02, 公開日: 2006-04-10, 最終更新日: 2024-05-15)

主引用文献

Amodeo, P.,Castiglione-Morelli, M.A.,Ostuni, A.,Battistuzzi, G.,Bavoso, A.
Structural Features in Eiav Ncp11: A Lentivirus Nucleocapsid Protein with a Short Linker
Biochemistry, 45:5517-, 2006

PubMed Abstract: Lentiviral nucleocapsid proteins are a class of multifunctional proteins that play an essential role in RNA packaging and viral infectivity. They contain two CX(2)CX(4)HX(4)C zinc binding motifs connected by a basic linker of variable length. The 3D structure of a 37-aa peptide corresponding to sequence 22-58 from lentiviral EIAV nucleocapsid protein NCp11, complexed with zinc, has been determined by 2D (1)H NMR spectroscopy, simulated annealing, and molecular dynamics. The solution structure consists of two zinc binding domains held together by a five-residue basic linker Arg(38)-Ala-Pro-Lys-Val(42) that allows for spatial proximity between the two finger domains. Observed linker folding is stabilized by H bonded secondary structure elements, resulting in an Omega-shaped central region, asymmetrically centered on the linker. The conformational differences and similarities with other NC zinc binding knuckles have been systematically analyzed. The two CCHC motifs, both characterized by a peculiar Pro-Gly sequence preceding the His residue, although preserving Zn-binding geometry and chirality of other known NC proteins, exhibit local fold differences both between each other and in comparison with other previously characterized retroviral CCHC motifs.

PubMed: 16634633
DOI: 10.1021/BI0524924

実験手法

SOLUTION NMR