2BJV

Crystal Structure of PspF(1-275) R168A mutant

Summary for 2BJV

• Entry DOI: 10.2210/pdb2bjv/pdb
• Related: 2BJW
• Descriptor: PSP OPERON TRANSCRIPTIONAL ACTIVATOR (2 entities in total)
• Functional Keywords: transcription, aaa, transcription activation, gene regulation, sigma54 activator, enhancer binding protein, pspf, atp-binding, dna- binding, transcription regulation
• Biological source: ESCHERICHIA COLI
• Cellular location: Cytoplasm : P37344
• Total number of polymer chains: 1
• Total formula weight: 29901.18

Authors

Rappas, M.,Schumacher, J.,Beuron, F.,Niwa, H.,Bordes, P.,Wigneshweraraj, S.,Keetch, C.A.,Robinson, C.V.,Buck, M.,Zhang, X. (deposition date: 2005-02-08, release date: 2005-03-31, Last modification date: 2024-05-08)

Primary citation

Rappas, M.,Schumacher, J.,Beuron, F.,Niwa, H.,Bordes, P.,Wigneshweraraj, S.,Keetch, C.A.,Robinson, C.V.,Buck, M.,Zhang, X.
Structural Insights Into the Activity of Enhancer-Binding Proteins
Science, 307:1972-, 2005

PubMed Abstract: Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.

PubMed: 15790859
DOI: 10.1126/SCIENCE.1105932

Experimental method

X-RAY DIFFRACTION (1.7 Å)