2BJQ

Crystal structure of the nematode sperm cell motility protein MFP2

2BJQ の概要

• エントリーDOI: 10.2210/pdb2bjq/pdb
• 関連するPDBエントリー: 1BJR
• 分子名称: MFP2A (2 entities in total)
• 機能のキーワード: motility, ascaris, nematode, msp
• 由来する生物種: ASCARIS SUUM
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38235.25

構造登録者

Grant, R.P.,Buttery, S.M.,Ekman, G.C.,Roberts, T.M.,Stewart, M. (登録日: 2005-02-07, 公開日: 2005-03-16, 最終更新日: 2024-05-08)

主引用文献

Grant, R.P.,Buttery, S.M.,Ekman, G.C.,Roberts, T.M.,Stewart, M.
Structure of Mfp2 and its Function in Enhancing Msp Polymerization in Ascaris Sperm Amoeboid Motility
J.Mol.Biol., 347:583-, 2005

PubMed Abstract: The simplicity and specialization of the cell motility machinery of Ascaris sperm provides a powerful system in which to probe the basic molecular mechanism of amoeboid cell motility. Although Ascaris sperm locomotion closely resembles that seen in many other types of crawling cell, movement is generated by modulation of a cytoskeleton based on the major sperm protein (MSP) rather than the actin present in other cell types. The Ascaris motility machinery can be studied conveniently in a cell-free in vitro system based on the movement of plasma membrane vesicles by fibres constructed from bundles of MSP filaments. In addition to ATP, MSP and a plasma membrane protein, reconstitution of MSP motility in this cell-free extract requires cytosolic proteins to orchestrate the site-specific assembly and bundling of MSP filaments that generates locomotion. One of these proteins, MFP2, accelerates the rate of movement in this assay. Here, we describe crystal structures of two isoforms of MFP2 and show that both are constructed from two domains that have the same fold based on a novel, compact beta sheet arrangement. Patterns of conservation observed in a structure-based analysis of MFP2 sequences from different nematode species identified regions that may be putative functional interfaces involved both in interactions between MFP2 domains and also with other components of the sperm motility machinery. Analysis of the growth of fibres in vitro in the presence of added MFP2 indicated that MFP2 increases the rate of locomotion by enhancing the effective rate of MSP filament polymerization. This observation, together with the structural data, suggests that MFP2 may function in a manner analogous to formins in actin-based motility.

PubMed: 15755452
DOI: 10.1016/J.JMB.2005.01.054

実験手法

X-RAY DIFFRACTION (1.75 Å)