2BJH

Crystal Structure Of S133A AnFaeA-ferulic acid complex

2BJH の概要

• エントリーDOI: 10.2210/pdb2bjh/pdb
• 関連するPDBエントリー: 1USW 1UWC 1UZA
• 分子名称: FERULOYL ESTERASE A, 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: degradation plant cell walls, feruloyl esterase, glycoprotein, hydrolase, serine esterase, xylan degradation
• 由来する生物種: ASPERGILLUS NIGER
• 細胞内の位置: Secreted: O42807
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 86941.42

構造登録者

Faulds, C.B.,Molina, R.,Gonzalez, R.,Husband, F.,Juge, N.,Sanz-Aparicio, J.,Hermoso, J.A. (登録日: 2005-02-02, 公開日: 2005-09-07, 最終更新日: 2024-10-23)

主引用文献

Faulds, C.B.,Molina, R.,Gonzalez, R.,Husband, F.,Juge, N.,Sanz-Aparicio, J.,Hermoso, J.A.
Probing the Determinants of Substrate Specificity of a Feruloyl Esterase, Anfaea, from Aspergillus Niger
FEBS J., 272:4362-, 2005

PubMed Abstract: Feruloyl esterases hydrolyse phenolic groups involved in the cross-linking of arabinoxylan to other polymeric structures. This is important for opening the cell wall structure making material more accessible to glycoside hydrolases. Here we describe the crystal structure of inactive S133A mutant of type-A feruloyl esterase from Aspergillus niger (AnFaeA) in complex with a feruloylated trisaccharide substrate. Only the ferulic acid moiety of the substrate is visible in the electron density map, showing interactions through its OH and OCH(3) groups with the hydroxyl groups of Tyr80. The importance of aromatic and polar residues in the activity of AnFaeA was also evaluated using site-directed mutagenesis. Four mutant proteins were heterologously expressed in Pichia pastoris, and their kinetic properties determined against methyl esters of ferulic, sinapic, caffeic and p-coumaric acid. The k(cat) of Y80S, Y80V, W260S and W260V was drastically reduced compared to that of the wild-type enzyme. However, the replacement of Tyr80 and Trp260 with smaller residues broadened the substrate specificity of the enzyme, allowing the hydrolysis of methyl caffeate. The role of Tyr80 and Trp260 in AnFaeA are discussed in light of the three-dimensional structure.

PubMed: 16128806
DOI: 10.1111/J.1742-4658.2005.04849.X

実験手法

X-RAY DIFFRACTION (2.54 Å)