2BJ9

NIKR with bound NICKEL and phosphate

2BJ9 の概要

• エントリーDOI: 10.2210/pdb2bj9/pdb
• 関連するPDBエントリー: 1Q5V 1Q5Y 2BJ1 2BJ3 2BJ7 2BJ8
• 分子名称: NICKEL RESPONSIVE REGULATOR, NICKEL (II) ION, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: transcription, repressor, pyrococcus horikoshii, nikr, dna-binding, metal-binding, nickel, transcription regulation, riken structural genomics/proteomics initiative, rsgi, structural genomics
• 由来する生物種: PYROCOCCUS HORIKOSHII
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32338.27

構造登録者

Tahirov, T.H.,Chivers, P.T. (登録日: 2005-01-31, 公開日: 2005-04-08, 最終更新日: 2024-05-08)

主引用文献

Chivers, P.T.,Tahirov, T.H.
Structure of Pyrococcus Horikoshii Nikr: Nickel Sensing and Implications for the Regulation of DNA Recognition
J.Mol.Biol., 348:597-, 2005

PubMed Abstract: The Pyrococcus horikoshii OT3 genome contains a gene (PH0601 or nikR) encoding a protein (PhNikR) that shares 33.8% amino acid sequence identity with Escherichia coli nickel responsive repressor NikR (EcNikR), including many residues that are functionally important in the E.coli ortholog. We succeeded in crystallization and structural characterization of PhNikR in the apo form and two nickel bound forms that exhibit different conformations, open and closed. Moreover, we have identified a putative "low-affinity" nickel-binding pocket in the closed form. This binding site has unusual nickel coordination and exhibits high sensitivity to phosphate in the crystal structure. Analysis of the PhNikR structures and structure-based mutational studies with EcNikR reveals a plausible mechanism of nickel-dependent promoter recognition by the NikR family of proteins.

PubMed: 15826657
DOI: 10.1016/J.JMB.2005.03.017

実験手法

X-RAY DIFFRACTION (3 Å)