2BJ6

Crystal Structure of a decameric HNA-RNA hybrid

Summary for 2BJ6

• Entry DOI: 10.2210/pdb2bj6/pdb
• Descriptor: SYNTHETIC HNA, 5'-R(*GP*GP*CP*AP*UP*UP*AP*CP*GP*GP)-3', SULFATE ION, ... (4 entities in total)
• Functional Keywords: nucleic acid (hna/rna), dna, rna, hna, hexitol nucleic acid, hybrid, duplex, modified nucleic acid, antisense, backbone modification, nucleic acid (hna-rna) complex
• Biological source: HOMO SAPIENS; More
• Total number of polymer chains: 8
• Total formula weight: 25929.20

Authors

Maier, T.,Przylas, I.,Straeter, N.,Herdewijn, P.,Saenger, W. (deposition date: 2005-01-30, release date: 2005-03-09, Last modification date: 2024-05-08)

Primary citation

Maier, T.,Przylas, I.,Strater, N.,Herdewijn, P.,Saenger, W.
Reinforced Hna Backbone Hydration in the Crystal Structure of a Decameric Hna/RNA Hybrid
J.Am.Chem.Soc., 127:2937-, 2005

PubMed Abstract: The crystal structure of a decameric HNA/RNA (HNA = 2',3'-dideoxy-1',5'-anhydro-d-arabinohexitol nucleic acid) hybrid with the RNA sequence 5'-GGCAUUACGG-3' is the first crystal structure of a hybrid duplex between a naturally occurring nucleic acid and a strand, which is fully modified to contain a six-membered ring instead of ribose. The presence of four duplex helices in the asymmetric unit allows for a detailed discussion of hydration, which revealed a tighter spinelike backbone hydration for the HNA- than for the RNA-strands. The reinforced backbone hydration is suggested to contribute significantly to the exceptional stability of HNA-containing duplexes and might be one of the causes for the evolutionary preference for ribose-derived nucleic acids.

PubMed: 15740130
DOI: 10.1021/JA045843V

Experimental method

X-RAY DIFFRACTION (2.6 Å)