2BIC

The solution structure of the recombinant elicitor protein PcF from the oomycete pathogen P. cactorum

2BIC の概要

• エントリーDOI: 10.2210/pdb2bic/pdb
• NMR情報: BMRB: 6520
• 分子名称: PHYTOTOXIC PROTEIN PCF (1 entity in total)
• 機能のキーワード: plant pathogen, phytophthora cactorum fragaria protein, oomycete plant pathogen, elicitin family, toxin
• 由来する生物種: PHYTOPHTHORA CACTORUM
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5617.18

構造登録者

Nicastro, G.,Orsomando, G.,Desario, F.,Ferrari, E.,Manconi, L.,Spisni, A.,Ruggieri, S. (登録日: 2005-01-20, 公開日: 2006-06-28, 最終更新日: 2024-10-09)

主引用文献

Nicastro, G.,Orsomando, G.,Ferrari, E.,Manconi, L.,Desario, F.,Amici, A.,Naso, A.,Carpaneto, A.,Pertinhez, T.A.,Ruggieri, S.,Spisni, A.
Solution Structure of the Phytotoxic Protein Pcf: The First Characterized Member of the Phytophthora Pcf Toxin Family.
Protein Sci., 18:1786-, 2009

PubMed Abstract: The PcF protein from Phytophthora cactorum is the first member of the "PcF toxin family" from the plant pathogens Phytophthora spp. It is able to induce withering in tomato and strawberry leaves. The lack of sequence similarity with other proteins hampers the identification of the molecular mechanisms responsible for its toxicity. Here, we show that the six cysteines form a disulphide pattern that is exclusive for PcF and essential for the protein withering activity. The NMR solution structure identifies a novel fold among protein effectors: a helix-loop-helix motif. The presence of a negatively charged surface suggests that it might act as a site of electrostatic interaction. Interestingly, a good fold match with Ole e 6, a plant protein with allergenic activity, highlighted the spatial superimposition of a stretch of identical residues. This finding suggests a possible biological activity based on molecular mimicry.

PubMed: 19554629
DOI: 10.1002/PRO.168

実験手法

SOLUTION NMR