2BGA

Bacillus cereus metallo-beta-lactamase (BcII) Arg (121) Cys mutant. Solved at pH7 using 20 Micromolar ZnSO4 in the buffer. 1mM DTT was used as a reducing agent. Cys221 is oxidized.

2BGA の概要

• エントリーDOI: 10.2210/pdb2bga/pdb
• 関連するPDBエントリー: 1BC2 1BMC 1BVT 1DXK 1MQO 2BC2 2BFK 2BFL 2BFZ 2BG2 2BG6 2BG7 2BG8 3BC2
• 分子名称: BETA-LACTAMASE II, AZIDE ION, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: hydrolase, zinc, metallo-beta-lactamase, antibiotic resistance
• 由来する生物種: BACILLUS CEREUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50910.36

構造登録者

Davies, A.M.,Rasia, R.M.,Vila, A.J.,Sutton, B.J.,Fabiane, S.M. (登録日: 2004-12-17, 公開日: 2005-03-31, 最終更新日: 2023-12-13)

• 主引用文献: Davies, A.M.,Rasia, R.M.,Vila, A.J.,Sutton, B.J.,Fabiane, S.M.; Effect of Ph on the Active Site of an Arg121Cys Mutant of the Metallo-Beta-Lactamase from Bacillus Cereus: Implications for the Enzyme Mechanism; Biochemistry, 44:4841-, 2005; PubMed: 15779910; DOI: 10.1021/BI047709T

実験手法

X-RAY DIFFRACTION (2.7 Å)