2BFZ

Bacillus cereus metallo-beta-lactamase (BcII) Arg (121) Cys mutant. Solved at pH4.5 using 20mM ZnSO4 in buffer. 1mM DTT was used as a reducing agent. Cys221 is oxidized.

2BFZ の概要

• エントリーDOI: 10.2210/pdb2bfz/pdb
• 関連するPDBエントリー: 2BG2 2BG6 2BG7 2BG8 2BGA
• 分子名称: BETA-LACTAMASE II, GLYCEROL, ZINC ION, ... (7 entities in total)
• 機能のキーワード: hydrolase, antibiotic resistance
• 由来する生物種: BACILLUS CEREUS; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51509.02

構造登録者

Davies, A.M.,Rasia, R.M.,Vila, A.J.,Sutton, B.J.,Fabiane, S.M. (登録日: 2004-12-16, 公開日: 2005-03-31, 最終更新日: 2023-12-13)

• 主引用文献: Davies, A.M.,Rasia, R.M.,Vila, A.J.,Sutton, B.J.,Fabiane, S.M.; Effect of Ph on the Active Site of an Arg121Cys Mutant of the Metallo-Beta-Lactamase from Bacillus Cereus: Implications for the Enzyme Mechanism; Biochemistry, 44:4841-, 2005; PubMed: 15779910; DOI: 10.1021/BI047709T

実験手法

X-RAY DIFFRACTION (2.3 Å)