2BFH

CRYSTAL STRUCTURE OF BASIC FIBROBLAST GROWTH FACTOR AT 1.6 ANGSTROMS RESOLUTION

2BFH の概要

• エントリーDOI: 10.2210/pdb2bfh/pdb
• 分子名称: BASIC FIBROBLAST GROWTH FACTOR (2 entities in total)
• 機能のキーワード: growth factor
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14670.92

構造登録者

Kitagawa, Y.,Ago, H.,Katsube, Y.,Fujishima, A.,Matsuura, Y. (登録日: 1993-08-31, 公開日: 1994-01-31, 最終更新日: 2024-02-14)

主引用文献

Ago, H.,Kitagawa, Y.,Fujishima, A.,Matsuura, Y.,Katsube, Y.
Crystal structure of basic fibroblast growth factor at 1.6 A resolution.
J.Biochem.(Tokyo), 110:360-363, 1991

PubMed Abstract: We have determined the crystal structures of two types of human basic fibroblast growth factor, the serine analogue and the wild-type, at 1.6 and 2.5 A resolution, respectively. Two good heavy atom derivatives were found and used for multiple isomorphous replacement phasing. The atomic coordinates were refined using the Hendrickson & Konnert program for stereochemically restrained refinement against structure factors. The crystallographic R factors were reduced to 15.3% for the serine analogue structure and 16.0% for the wild-type structure. The serine analogue and wild-type structures have been found to be almost identical, the root-mean-square deviation between the corresponding C alpha atoms being 0.11 A. Their structures are composed of twelve beta-strands forming a barrel and three loops. Their molecules have an approximate threefold internal symmetry and are similar in architecture to that of interleukin-1 beta. A possible heparin-binding site, which comprises five basic residues, Lys119, Arg120, Lys125, Lys129, and Lys135, has been revealed by calculating the electrostatic potential energy.

PubMed: 1769963

実験手法

X-RAY DIFFRACTION (2.5 Å)