2BEZ

Structure of a proteolitically resistant core from the severe acute respiratory syndrome coronavirus S2 fusion protein

2BEZ の概要

• エントリーDOI: 10.2210/pdb2bez/pdb
• 関連するPDBエントリー: 2BEQ
• 分子名称: Spike glycoprotein, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: coiled coil, membrane fusion, severe acute respiratory syndrome, viral protein
• 由来する生物種: Human SARS coronavirus (SARS-CoV); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13072.48

構造登録者

Supekar, V.M.,Bruckmann, C.,Ingallinella, P.,Bianchi, E.,Pessi, A.,Carfi, A. (登録日: 2004-12-02, 公開日: 2004-12-22, 最終更新日: 2024-05-08)

主引用文献

Supekar, V.M.,Bruckmann, C.,Ingallinella, P.,Bianchi, E.,Pessi, A.,Carfi, A.
Structure of a Proteolytically Resistant Core from the Severe Acute Respiratory Syndrome Coronavirus S2 Fusion Protein
Proc.Natl.Acad.Sci.USA, 101:17958-, 2004

PubMed Abstract: A coronavirus (CoV) has recently been identified as the causative agent of the severe acute respiratory syndrome (SARS) in humans. CoVs enter target cells through fusion of viral and cellular membranes mediated by the viral envelope glycoprotein S. We have determined by x-ray crystallography the structure of a proteolytically stable core fragment from the heptad repeat (HR) regions HR1 and HR2 of the SARS-CoV S protein. We have also determined the structure of an HR1-HR2 S core fragment, containing a shorter HR1 peptide and a C-terminally longer HR2 peptide that extends up to the transmembrane region. In these structures, three HR1 helices form a parallel coiled-coil trimer, whereas three HR2 peptides pack in an oblique and antiparallel fashion into the coiled-coil hydrophobic grooves, adopting mixed extended and alpha-helical conformations as in postfusion paramyxoviruses F proteins structures. Our structure positions a previously proposed internal fusion peptide adjacent to the N-terminus of HR1. Peptides from the HR2 region of SARS-CoV S have been shown to inhibit viral entry and infection in vitro. The structures presented here can thus open the path to the design of small-molecule inhibitors of viral entry and candidate vaccine antigens against this virus.

PubMed: 15604146
DOI: 10.1073/PNAS.0406128102

実験手法

X-RAY DIFFRACTION (1.6 Å)