2BDP

CRYSTAL STRUCTURE OF BACILLUS DNA POLYMERASE I FRAGMENT COMPLEXED TO 9 BASE PAIRS OF DUPLEX DNA

2BDP の概要

• エントリーDOI: 10.2210/pdb2bdp/pdb
• 分子名称: DNA (5'-D(*GP*CP*AP*TP*GP*AP*TP*GP*C)-3'), DNA (5'-D(P*AP*GP*CP*AP*TP*CP*AP*TP*GP*C)-3'), PROTEIN (DNA POLYMERASE I), ... (6 entities in total)
• 機能のキーワード: bacillus stearothermophilus dna polymerase, bf thermophilus polymerase, complex (nucleotidyltransferase-dna), transferase-dna complex, transferase/dna
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 72298.18

構造登録者

Kiefer, J.R.,Mao, C.,Beese, L.S. (登録日: 1997-11-17, 公開日: 1999-01-13, 最終更新日: 2024-04-03)

主引用文献

Kiefer, J.R.,Mao, C.,Braman, J.C.,Beese, L.S.
Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal.
Nature, 391:304-307, 1998

PubMed Abstract: DNA polymerases copy DNA templates with remarkably high fidelity, checking for correct base-pair formation both at nucleotide insertion and at subsequent DNA extension steps. Despite extensive biochemical, genetic and structural studies, the mechanism by which nucleotides are correctly incorporated is not known. Here we present high-resolution crystal structures of a thermostable bacterial (Bacillus stearothermophilus) DNA polymerase I large fragments with DNA primer templates bound productively at the polymerase active site. The active site retains catalytic activity, allowing direct observation of the products of several rounds of nucleotide incorporation. The polymerase also retains its ability to discriminate between correct and incorrectly paired nucleotides in the crystal. Comparison of the structures of successively translocated complexes allows the structural features for the sequence-independent molecular recognition of correctly formed base pairs to be deduced unambiguously. These include extensive interactions with the first four to five base pairs in the minor groove, location of the terminal base pair in a pocket of excellent steric complementarity favouring correct base-pair formation, and a conformational switch from B-form to underwound A-form DNA at the polymerase active site.

PubMed: 9440698
DOI: 10.1038/34693

実験手法

X-RAY DIFFRACTION (1.8 Å)