2BB8

N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE

2BB8 の概要

• エントリーDOI: 10.2210/pdb2bb8/pdb
• 分子名称: INTEGRASE (1 entity in total)
• 機能のキーワード: dna recombination, integrase, dna binding, transposition, transposase
• 由来する生物種: Enterococcus faecalis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8462.62

構造登録者

Clubb, R.T.,Connolly, K.M.,Wojciak, J.M. (登録日: 1998-05-12, 公開日: 1998-11-25, 最終更新日: 2024-05-22)

主引用文献

Connolly, K.M.,Wojciak, J.M.,Clubb, R.T.
Site-specific DNA binding using a variation of the double stranded RNA binding motif.
Nat.Struct.Biol., 5:546-550, 1998

PubMed Abstract: The integrase family of site-specific recombinases catalyze a diverse array of DNA rearrangements in archaebacteria, eubacteria and yeast. The solution structure of the DNA binding domain of the integrase protein from the conjugative transposon Tn916 has been determined using NMR spectroscopy. The structure provides the first insights into distal site DNA binding by a site-specific integrase and reveals that the N-terminal domain is structurally similar to the double stranded RNA binding domain (dsRBD). The results of chemical shift mapping experiments suggest that the integrase protein interacts with DNA using residues located on the face of its three stranded beta-sheet. This surface differs from the proposed RNA binding surface in dsRBDs, suggesting that different surfaces on the same protein fold can be used to bind DNA and RNA.

PubMed: 9665166
DOI: 10.1038/799

実験手法

SOLUTION NMR