2BAF

Bovine Fibrinogen alpha-C Domain

2BAF の概要

• エントリーDOI: 10.2210/pdb2baf/pdb
• NMR情報: BMRB: 6893
• 分子名称: Fibrinogen alpha chain (1 entity in total)
• 機能のキーワード: fibrinogen, beta hairpin, blood clotting
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P02672
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18152.63

構造登録者

Burton, R.A. (登録日: 2005-10-14, 公開日: 2006-02-28, 最終更新日: 2024-10-16)

主引用文献

Burton, R.A.,Tsurupa, G.,Medved, L.,Tjandra, N.
Identification of an Ordered Compact Structure within the Recombinant Bovine Fibrinogen alphaC-Domain Fragment by NMR.
Biochemistry, 45:2257-2266, 2006

PubMed Abstract: The NMR solution structure of the bovine fibrinogen alphaC-domain fragment, including residues Aalpha374-538, reveals a type-I' beta-hairpin, restricted at the base by a C423-C453 disulfide linkage and a short turn preceding C423. Although both faces of the hairpin are formed mainly by hydrophilic residues, one of them is uncharged while the other has a characteristic pattern of charged residues which are highly conserved among vertebrate species. Chemical shift indexing and relaxation data indicate the presence of a collapsed hydrophobic region next to the hairpin that includes approximately 30 residues with slower concerted motion and higher content of nonpolar residues and, according to a previous study (Tsurupa, G., Tsonev, L., and Medved, L. (2002) Biochemistry 41, 6449-6459), may cooperate with the hairpin to form a compact cooperative unit (domain). Structure and relaxation data show that the region between C423 and C453 is populated by both random coil and beta-structure, suggesting that the cooperative structure in the isolated alphaC-domain is intrinsically unstable. This observation is in agreement with a very low energy of stabilization of the Aalpha374-538 fragment determined in unfolding experiments. The low stability of the alphaC-domain suggests a possible explanation for the previously observed intra- and intermolecular interactions of these domains in fibrinogen and fibrin.

PubMed: 16475814
DOI: 10.1021/bi052380c

実験手法

SOLUTION NMR