2BA2
Crystal structure of the DUF16 domain of MPN010 from Mycoplasma pneumoniae
Summary for 2BA2
| Entry DOI | 10.2210/pdb2ba2/pdb |
| Descriptor | Hypothetical UPF0134 protein MPN010 (2 entities in total) |
| Functional Keywords | duf16, mpn010, hypothetical protein, coiled-coil, stutter, structural genomics, psi, protein structure initiative, berkeley structural genomics center, bsgc, unknown function |
| Biological source | Mycoplasma pneumoniae |
| Total number of polymer chains | 3 |
| Total formula weight | 29367.33 |
| Authors | Shin, D.H.,Kim, J.-S.,Yokota, H.,Kim, R.,Kim, S.-H.,Berkeley Structural Genomics Center (BSGC) (deposition date: 2005-10-13, release date: 2006-03-07, Last modification date: 2024-02-14) |
| Primary citation | Shin, D.H.,Kim, J.-S.,Yokota, H.,Kim, R.,Kim, S.-H. Crystal structure of the DUF16 domain of MPN010 from Mycoplasma pneumoniae. Protein Sci., 15:921-928, 2006 Cited by PubMed Abstract: We have determined the crystal structure of the DUF16 domain of unknown function encoded by the gene MPN010 of Mycoplasma pneumoniae at 1.8 A resolution. The crystal structure revealed that this domain is composed of two separated homotrimeric coiled-coils. The shorter one consists of 11 highly conserved residues. The sequence comprises noncanonical heptad repeats that induce a right-handed coiled-coil structure. The longer one is composed of approximately nine heptad repeats. In this coiled-coil structure, there are three distinguishable regions that confer unique structural properties compared with other known homotrimeric coiled-coils. The first part, containing one stutter, is an unusual phenylalanine-rich region that is not found in any other coiled-coil structures. The second part is a highly conserved glutamine-rich region, frequently found in other trimeric coiled-coil structures. The last part is composed of prototype heptad repeats. The phylogenetic analysis of the DUF16 family together with a secondary structure prediction shows that the DUF16 family can be classified into five subclasses according to N-terminal sequences. Based on the structural comparison with other coiled-coil structures, a probable molecular function of the DUF16 family is discussed. PubMed: 16522803DOI: 10.1110/ps.051993506 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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