2BA2

Crystal structure of the DUF16 domain of MPN010 from Mycoplasma pneumoniae

2BA2 の概要

• エントリーDOI: 10.2210/pdb2ba2/pdb
• 分子名称: Hypothetical UPF0134 protein MPN010 (2 entities in total)
• 機能のキーワード: duf16, mpn010, hypothetical protein, coiled-coil, stutter, structural genomics, psi, protein structure initiative, berkeley structural genomics center, bsgc, unknown function
• 由来する生物種: Mycoplasma pneumoniae
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 29367.33

構造登録者

Shin, D.H.,Kim, J.-S.,Yokota, H.,Kim, R.,Kim, S.-H.,Berkeley Structural Genomics Center (BSGC) (登録日: 2005-10-13, 公開日: 2006-03-07, 最終更新日: 2024-02-14)

主引用文献

Shin, D.H.,Kim, J.-S.,Yokota, H.,Kim, R.,Kim, S.-H.
Crystal structure of the DUF16 domain of MPN010 from Mycoplasma pneumoniae.
Protein Sci., 15:921-928, 2006

PubMed Abstract: We have determined the crystal structure of the DUF16 domain of unknown function encoded by the gene MPN010 of Mycoplasma pneumoniae at 1.8 A resolution. The crystal structure revealed that this domain is composed of two separated homotrimeric coiled-coils. The shorter one consists of 11 highly conserved residues. The sequence comprises noncanonical heptad repeats that induce a right-handed coiled-coil structure. The longer one is composed of approximately nine heptad repeats. In this coiled-coil structure, there are three distinguishable regions that confer unique structural properties compared with other known homotrimeric coiled-coils. The first part, containing one stutter, is an unusual phenylalanine-rich region that is not found in any other coiled-coil structures. The second part is a highly conserved glutamine-rich region, frequently found in other trimeric coiled-coil structures. The last part is composed of prototype heptad repeats. The phylogenetic analysis of the DUF16 family together with a secondary structure prediction shows that the DUF16 family can be classified into five subclasses according to N-terminal sequences. Based on the structural comparison with other coiled-coil structures, a probable molecular function of the DUF16 family is discussed.

PubMed: 16522803
DOI: 10.1110/ps.051993506

実験手法

X-RAY DIFFRACTION (1.8 Å)