2B9Z

Solution structure of FHV B2, a viral suppressor of RNAi

Summary for 2B9Z

• Entry DOI: 10.2210/pdb2b9z/pdb
• Descriptor: B2 protein (1 entity in total)
• Functional Keywords: symmetric antiparallel homodimer, all alpha-helical, viral protein
• Biological source: Flock house virus
• Total number of polymer chains: 2
• Total formula weight: 16126.76

Authors

Lingel, A.,Simon, B.,Izaurralde, E.,Sattler, M. (deposition date: 2005-10-13, release date: 2005-11-29, Last modification date: 2024-05-22)

Primary citation

Lingel, A.,Simon, B.,Izaurralde, E.,Sattler, M.
The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition.
Embo Rep., 6:1149-1155, 2005

PubMed Abstract: We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three alpha-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (alpha2/alpha2') forms an elongated binding interface with double-stranded RNA (dsRNA). This implies a novel mode of dsRNA recognition and provides insights into the mechanism of RNAi suppression by B2.

PubMed: 16270100
DOI: 10.1038/sj.embor.7400583

Experimental method

SOLUTION NMR