2B7Y

Fava Bean Lectin-Glucose Complex

2B7Y の概要

• エントリーDOI: 10.2210/pdb2b7y/pdb
• 分子名称: Favin beta chain, Favin alpha chain, alpha-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: lectin-glucose complex, plant lectin, glycoprotein, carbohydrate binding protein, d-glucose, protein-carbohydrate complex, lectin
• 由来する生物種: Vicia faba (fava bean); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 52085.50

構造登録者

Reeke Jr., G.N.,Becker, J.W. (登録日: 2005-10-05, 公開日: 2005-10-18, 最終更新日: 2024-11-13)

主引用文献

Reeke Jr., G.N.,Becker, J.W.
Three-dimensional structure of favin: saccharide binding-cyclic permutation in leguminous lectins
Science, 234:1108-1111, 1986

PubMed Abstract: The three-dimensional structure of favin, the glucose- and mannose-binding lectin of Vicia faba (vetch, broad bean), has been determined at a resolution of 2.8 angstroms by molecular replacement. The crystals contain specifically bound glucose and provide the first high-resolution view of specific saccharide binding in a leguminous lectin. The structure is similar to those of concanavalin A (Con A) and green pea lectin; differences from Con A show that minimal changes are needed to accommodate the cyclic permutation in amino acid sequence between the two molecules. The molecule is an ellipsoidal dimer dominated by extensive beta structures. Each protomer contains binding sites for two divalent metal ions (Mn2+ and Ca2+) and a specific saccharide. Glucose is bound by favin in a cleft in the molecular surface and has noncovalent contacts primarily with two peptide loops, one of which contains several metal ion ligands. The specific carbohydrate-binding site is similar to that of Con A in location and general peptide folding, despite several differences in specific amino acid residues.

PubMed: 3775378

実験手法

X-RAY DIFFRACTION (3 Å)