2B7V

Structure of ADAR2 dsRBM2

2B7V の概要

• エントリーDOI: 10.2210/pdb2b7v/pdb
• 関連するPDBエントリー: 2B7T
• 分子名称: Double-stranded RNA-specific editase 1 (1 entity in total)
• 機能のキーワード: rna editing, rna-binding protein, hydrolase
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7703.83

構造登録者

Stefl, R.,Xu, M.,Skrisovska, L.,Emeson, R.B.,Allain, F.H.-T. (登録日: 2005-10-05, 公開日: 2006-03-14, 最終更新日: 2024-05-22)

主引用文献

Stefl, R.,Xu, M.,Skrisovska, L.,Emeson, R.B.,Allain, F.H.-T.
Structure and specific RNA binding of ADAR2 double-stranded RNA binding motifs.
Structure, 14:345-355, 2006

PubMed Abstract: Adenosine deaminases that act on RNA (ADARs) site-selectively modify adenosines to inosines within RNA transcripts, thereby recoding genomic information. How ADARs select specific adenosine moieties for deamination is poorly understood. Here, we report NMR structures of the two double-stranded RNA binding motifs (dsRBMs) of rat ADAR2 and an NMR chemical shift perturbation study of the interaction of the two dsRBMs with a 71 nucleotide RNA encoding the R/G site of the GluR-B. We have identified the protein and the RNA surfaces involved in complex formation, allowing us to present an NMR-based model of the complex. We have found that dsRBM1 recognizes a conserved pentaloop, whereas dsRBM2 recognizes two bulged bases adjacent to the editing site, demonstrating RNA structure-dependent recognition by the ADAR2 dsRBMs. In vitro mutagenesis studies with both the protein and the RNA further support our structural findings.

PubMed: 16472753
DOI: 10.1016/j.str.2005.11.013

実験手法

SOLUTION NMR