2B5X

Solution Structure of a Thioredoxin-like Protein in the Reduced Form

2B5X の概要

• エントリーDOI: 10.2210/pdb2b5x/pdb
• 分子名称: YkuV protein (1 entity in total)
• 機能のキーワード: thioredoxin-like, oxidoreductase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17040.28

構造登録者

Zhang, X.,Xia, B.,Jin, C. (登録日: 2005-09-29, 公開日: 2006-01-17, 最終更新日: 2024-05-29)

主引用文献

Zhang, X.,Hu, Y.,Guo, X.,Lescop, E.,Li, Y.,Xia, B.,Jin, C.
The bacillus subtilis YKUV is a thiol-disulfide oxidoreductase revealed by its redox structures and activity
J.Biol.Chem., 281:8296-8304, 2006

PubMed Abstract: The Bacillus subtilis YkuV responds to environmental oxidative stress and plays an important role for the bacteria to adapt to the environment. Bioinformatic analysis suggests that YkuV is a homolog of membrane-anchored proteins and belongs to the thioredoxin-like protein superfamily containing the typical Cys-Xaa-Xaa-Cys active motif. However, the biological function of this protein remains unknown thus far. In order to elucidate the biological function, we have determined the solution structures of both the oxidized and reduced forms of B. subtilis YkuV by NMR spectroscopy and performed biochemical studies. Our results demonstrated that the reduced YkuV has a low midpoint redox potential, allowing it to reduce a variety of protein substrates. The overall structures of both oxidized and reduced forms are similar, with a typical thioredoxin-like fold. However, significant conformational changes in the Cys-Xaa-Xaa-Cys active motif of the tertiary structures are observed between the two forms. In addition, the backbone dynamics provide further insights in understanding the strong redox potential of the reduced YkuV. Furthermore, we demonstrated that YkuV is able to reduce different protein substrates in vitro. Together, our results clearly established that YkuV may function as a general thiol:disulfide oxidoreductase, which acts as an alternative for thioredoxin or thioredoxin reductase to maintain the reducing environment in the cell cytoplasm.

PubMed: 16418167
DOI: 10.1074/jbc.M512015200

実験手法

SOLUTION NMR