2B1O

Solution Structure of Ca2+-bound DdCAD-1

Summary for 2B1O

• Entry DOI: 10.2210/pdb2b1o/pdb
• Related: 1YHP
• NMR Information: BMRB: 6159
• Descriptor: Calcium-dependent cell adhesion molecule-1, CALCIUM ION (2 entities in total)
• Functional Keywords: ddcad-1, calcium, cell adherison, cell adhesion
• Biological source: Dictyostelium discoideum
• Cellular location: Cell membrane: P54657
• Total number of polymer chains: 1
• Total formula weight: 23938.78

Authors

Lin, Z.,Sriskanthadevan, S.,Huang, H.B.,Siu, C.H.,Yang, D.W. (deposition date: 2005-09-16, release date: 2006-09-26, Last modification date: 2024-05-29)

Primary citation

Lin, Z.,Sriskanthadevan, S.,Huang, H.B.,Siu, C.H.,Yang, D.W.
Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca(2+)-dependent cell-cell adhesion
Nat.Struct.Mol.Biol., 13:1016-1022, 2006

PubMed Abstract: DdCAD-1 is a novel Ca(2+)-dependent cell adhesion molecule that lacks a hydrophobic signal peptide and a transmembrane domain. DdCAD-1 is expressed by the social amoeba Dictyostelium discoideum at the onset of development. It is synthesized as a soluble protein and then transported to the plasma membrane by contractile vacuoles. Here we describe the novel features of the solution structures of Ca(2+)-free and Ca(2+)-bound monomeric DdCAD-1. DdCAD-1 contains two beta-sandwich domains, belonging to the betagamma-crystallin and immunoglobulin fold classes, respectively. Whereas the N-terminal domain has a major role in homophilic binding, the C-terminal domain tethers the protein to the cell membrane. From structural and mutational analyses, we propose a model for the Ca(2+)-bound DdCAD-1 dimer as a basis for understanding DdCAD-1-mediated cell-cell adhesion at the molecular level. Our results provide new insights into Ca(2+)-dependent mechanisms for cell-cell adhesion.

PubMed: 17057715
DOI: 10.1038/nsmb1162

Experimental method

SOLUTION NMR