2B0R

Crystal Structure of Cyclase-Associated Protein from Cryptosporidium parvum

2B0R の概要

• エントリーDOI: 10.2210/pdb2b0r/pdb
• 分子名称: possible adenyl cyclase-associated protein, UNKNOWN ATOM OR ION (2 entities in total)
• 機能のキーワード: structural genomics consortium, cryptosporidium parvum, cyclase-associated protein, sgc, unknown function
• 由来する生物種: Cryptosporidium parvum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44973.43

構造登録者

Tempel, W.,Dong, A.,Zhao, Y.,Lew, J.,Kozieradzki, I.,Alam, Z.,Melone, M.,Wasney, G.,Vedadi, M.,Arrowsmith, C.,Edwards, A.,Weigelt, J.,Sundstrom, M.,Hui, R.,Bochkarev, A.,Artz, J.,Structural Genomics Consortium (SGC) (登録日: 2005-09-14, 公開日: 2005-09-27, 最終更新日: 2023-08-23)

主引用文献

Hliscs, M.,Sattler, J.,Tempel, W.,Artz, J.D.,Dong, A.,Hui, R.,Matuschewski, K.,Schuler, H.
Structure and function of a G-actin sequestering protein with a vital role in malaria oocyst development inside the mosquito vector
J.Biol.Chem., 151:100-110, 2010

PubMed Abstract: Cyclase-associated proteins (CAPs) are evolutionary conserved G-actin-binding proteins that regulate microfilament turnover. CAPs have a modular structure consisting of an N-terminal adenylate cyclase binding domain, a central proline-rich segment, and a C-terminal actin binding domain. Protozoan parasites of the phylum Apicomplexa, such as Cryptosporidium and the malaria parasite Plasmodium, express small CAP orthologs with homology to the C-terminal actin binding domain (C-CAP). Here, we demonstrate by reverse genetics that C-CAP is dispensable for the pathogenic Plasmodium blood stages. However, c-cap(-) parasites display a complete defect in oocyst development in the insect vector. By trans-species complementation we show that the Cryptosporidium parvum ortholog complements the Plasmodium gene functions. Purified recombinant C. parvum C-CAP protein binds actin monomers and prevents actin polymerization. The crystal structure of C. parvum C-CAP shows two monomers with a right-handed beta-helical fold intercalated at their C termini to form the putative physiological dimer. Our results reveal a specific vital role for an apicomplexan G-actin-binding protein during sporogony, the parasite replication phase that precedes formation of malaria transmission stages. This study also exemplifies how Plasmodium reverse genetics combined with biochemical and structural analyses of orthologous proteins can offer a fast track toward systematic gene characterization in apicomplexan parasites.

PubMed: 20083609
DOI: 10.1074/jbc.M109.054916

実験手法

X-RAY DIFFRACTION (2.6 Å)