2AXY

Crystal Structure of KH1 domain of human Poly(C)-binding protein-2 with C-rich strand of human telomeric DNA

2AXY の概要

• エントリーDOI: 10.2210/pdb2axy/pdb
• 分子名称: C-rich strand of human telomeric dna, Poly(rC)-binding protein 2 (3 entities in total)
• 機能のキーワード: protein-dna complex, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q15366
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 40930.00

構造登録者

Du, Z.,Lee, J.K.,Tjhen, R.J.,Li, S.,Stroud, R.M.,James, T.L. (登録日: 2005-09-06, 公開日: 2005-09-27, 最終更新日: 2024-11-20)

主引用文献

Du, Z.,Lee, J.K.,Tjhen, R.,Li, S.,Pan, H.,Stroud, R.M.,James, T.L.
Crystal Structure of the First KH Domain of Human Poly(C)-binding Protein-2 in Complex with a C-rich Strand of Human Telomeric DNA at 1.7 A
J.Biol.Chem., 280:38823-38830, 2005

PubMed Abstract: Recognition of poly(C) DNA and RNA sequences in mammalian cells is achieved by a subfamily of the KH (hnRNP K homology) domain-containing proteins known as poly(C)-binding proteins (PCBPs). To reveal the molecular basis of poly(C) sequence recognition, we have determined the crystal structure, at 1.7-A resolution, of PCBP2 KH1 in complex with a 7-nucleotide DNA sequence (5'-AACCCTA-3') corresponding to one repeat of the human C-rich strand telomeric DNA. The protein-DNA interaction is mediated by the combination of several stabilizing forces including hydrogen bonding, electrostatic interactions, van der Waals contacts, and shape complementarities. Specific recognition of the three cytosine residues is realized by a dense network of hydrogen bonds involving the side chains of two conserved lysines and one glutamic acid. The co-crystal structure also reveals a protein-protein dimerization interface of PCBP2 KH1 located on the opposite side of the protein from the DNA binding groove. Numerous stabilizing protein-protein interactions, including hydrophobic contacts, stacking of aromatic side chains, and a large number of hydrogen bonds, indicate that the protein-protein interaction interface is most likely genuine. Interaction of PCBP2 KH1 with the C-rich strand of human telomeric DNA suggests that PCBPs may participate in mechanisms involved in the regulation of telomere/telomerase functions.

PubMed: 16186123
DOI: 10.1074/jbc.M508183200

実験手法

X-RAY DIFFRACTION (1.7 Å)