2AXT

Crystal Structure of Photosystem II from Thermosynechococcus elongatus

2AXT の概要

• エントリーDOI: 10.2210/pdb2axt/pdb
• 分子名称: Photosystem Q(B) protein, Photosystem II reaction center protein K, Photosystem II reaction center L protein, ... (33 entities in total)
• 機能のキーワード: photosystem, ps ii, ps2, membrane complex, transmembrane alpha-helix, electron transport
• 由来する生物種: Thermosynechococcus elongatus; 詳細
• 細胞内の位置: Cellular thylakoid membrane ; Multi-pass membrane protein : P0A445 Q8DHJ2; Cellular thylakoid membrane ; Single-pass membrane protein : Q9F1K9 Q8DIN8 Q8DHA7 Q8DIQ0 Q8DJ43 Q8DJZ6 P59087; Cellular thylakoid membrane ; Peripheral membrane protein ; Lumenal side : P0A431 Q9F1L5; Cellular thylakoid membrane {ECO:0000255|HAMAP-Rule:MF_01378, ECO:0000269|PubMed:12881497, ECO:0000269|PubMed:14764885, ECO:0000269|PubMed:16355230, ECO:0000269|PubMed:17935689, ECO:0000269|PubMed:19219048, ECO:0000269|PubMed:20558739, ECO:0000269|PubMed:21367867, ECO:0000269|PubMed:22665786, ECO:0000269|PubMed:23413188, ECO:0000269|PubMed:25006873, ECO:0000269|PubMed:25043005, ECO:0000269|Ref: P0A386; Cellular thylakoid membrane {ECO:0000255|HAMAP-Rule:MF_00642, ECO:0000269|PubMed:14764885, ECO:0000269|PubMed:16049768, ECO:0000269|PubMed:16172937, ECO:0000269|PubMed:16355230, ECO:0000269|PubMed:17935689, ECO:0000269|PubMed:19219048, ECO:0000269|PubMed:20558739, ECO:0000269|PubMed:21367867, ECO:0000269|PubMed:22665786, ECO:0000269|PubMed:23413188, ECO:0000269|PubMed:25006873, ECO:0000269|PubMed:25043005, ECO:0000269|Ref: Q8DIP0; Cellular thylakoid membrane {ECO:0000255|HAMAP-Rule:MF_00643, ECO:0000269|PubMed:14764885, ECO:0000269|PubMed:16355230, ECO:0000269|PubMed:17935689, ECO:0000269|PubMed:19219048, ECO:0000269|PubMed:20558739, ECO:0000269|PubMed:21367867, ECO:0000269|PubMed:22665786, ECO:0000269|PubMed:23413188, ECO:0000269|PubMed:25006873, ECO:0000269|PubMed:25043005, ECO:0000269|Ref: Q8DIN9
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 720870.76

構造登録者

Loll, B.,Kern, J.,Saenger, W.,Zouni, A.,Biesiadka, J. (登録日: 2005-09-06, 公開日: 2005-12-27, 最終更新日: 2025-02-05)

主引用文献

Loll, B.,Kern, J.,Saenger, W.,Zouni, A.,Biesiadka, J.
Towards complete cofactor arrangement in the 3.0 A resolution structure of photosystem II
NATURE, 438:1040-1044, 2005

PubMed Abstract: Oxygenic photosynthesis in plants, algae and cyanobacteria is initiated at photosystem II, a homodimeric multisubunit protein-cofactor complex embedded in the thylakoid membrane. Photosystem II captures sunlight and powers the unique photo-induced oxidation of water to atmospheric oxygen. Crystallographic investigations of cyanobacterial photosystem II have provided several medium-resolution structures (3.8 to 3.2 A) that explain the general arrangement of the protein matrix and cofactors, but do not give a full picture of the complex. Here we describe the most complete cyanobacterial photosystem II structure obtained so far, showing locations of and interactions between 20 protein subunits and 77 cofactors per monomer. Assignment of 11 beta-carotenes yields insights into electron and energy transfer and photo-protection mechanisms in the reaction centre and antenna subunits. The high number of 14 integrally bound lipids reflects the structural and functional importance of these molecules for flexibility within and assembly of photosystem II. A lipophilic pathway is proposed for the diffusion of secondary plastoquinone that transfers redox equivalents from photosystem II to the photosynthetic chain. The structure provides information about the Mn4Ca cluster, where oxidation of water takes place. Our study uncovers near-atomic details necessary to understand the processes that convert light to chemical energy.

PubMed: 16355230
DOI: 10.1038/nature04224

実験手法

X-RAY DIFFRACTION (3 Å)