2AXR

Crystal structure of glucooligosaccharide oxidase from Acremonium strictum: a novel flavinylation of 6-S-cysteinyl, 8alpha-N1-histidyl FAD

2AXR の概要

• エントリーDOI: 10.2210/pdb2axr/pdb
• 関連するPDBエントリー: 1ZR6
• 分子名称: glucooligosaccharide oxidase, 2-acetamido-2-deoxy-beta-D-glucopyranose, ZINC ION, ... (6 entities in total)
• 機能のキーワード: alpha+beta, covalent flavoenzyme, oxidoreductase
• 由来する生物種: Acremonium strictum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57786.34

構造登録者

Huang, C.-H.,Lai, W.-L.,Vasella, A.,Tsai, Y.-C.,Liaw, S.-H. (登録日: 2005-09-05, 公開日: 2005-09-13, 最終更新日: 2024-11-20)

主引用文献

Huang, C.-H.,Lai, W.-L.,Lee, M.H.,Chen, C.J.,Vasella, A.,Tsai, Y.-C.,Liaw, S.-H.
Crystal Structure of Glucooligosaccharide Oxidase from Acremonium strictum: A NOVEL FLAVINYLATION OF 6-S-CYSTEINYL, 8{alpha}-N1-HISTIDYL FAD
J.Biol.Chem., 280:38831-38838, 2005

PubMed Abstract: Glucooligosaccharide oxidase from Acremonium strictum has been screened for potential applications in oligosaccharide acid production and alternative carbohydrate detection, because it catalyzes the oxidation of glucose, maltose, lactose, cellobiose and cello- and maltooligosaccharides. We report the crystal structures of the enzyme and of its complex with an inhibitor, 5-amino-5-deoxy- cellobiono-1,5-lactam at 1.55- and 1.98-A resolution, respectively. Unexpectedly, the protein structure demonstrates the first known double attachment flavinylation, 6-S-cysteinyl, 8alpha-N1-histidyl FAD. The FAD cofactor is cross-linked to the enzyme via the C(6) atom and the 8alpha-methyl group of the isoalloxazine ring with Cys(130) and His(70), respectively. This sugar oxidase possesses an open carbohydrate-binding groove, allowing the accommodation of higher oligosaccharides. The complex structure suggests that this enzyme may prefer a beta-d-glucosyl residue at the reducing end with the conserved Tyr(429) acting as a general base to abstract the OH(1) proton in concert with the H(1) hydride transfer to the flavin N(5). Finally, a detailed comparison illustrates the structural conservation as well as the divergence between this protein and its related flavoenzymes.

PubMed: 16154992
DOI: 10.1074/jbc.M506078200

実験手法

X-RAY DIFFRACTION (1.98 Å)