2AV8

Y122F MUTANT OF RIBONUCLEOTIDE REDUCTASE FROM ESCHERICHIA COLI

2AV8 の概要

• エントリーDOI: 10.2210/pdb2av8/pdb
• 分子名称: RIBONUCLEOTIDE REDUCTASE R2, FE (II) ION, MU-OXO-DIIRON, ... (4 entities in total)
• 機能のキーワード: oxidoreductase, dna replication
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79486.99

構造登録者

Han, S.,Arvai, A.,Tainer, J.A. (登録日: 1997-09-30, 公開日: 1998-10-28, 最終更新日: 2024-05-22)

主引用文献

Tong, W.,Burdi, D.,Riggs-Gelasco, P.,Chen, S.,Edmondson, D.,Huynh, B.H.,Stubbe, J.,Han, S.,Arvai, A.,Tainer, J.A.
Characterization of Y122F R2 of Escherichia coli ribonucleotide reductase by time-resolved physical biochemical methods and X-ray crystallography.
Biochemistry, 37:5840-5848, 1998

PubMed Abstract: Ribonucleotide reductase (RNR) from Escherichia coli catalyzes the conversion of ribonucleotides to deoxyribonucleotides. It is composed of two homodimeric subunits, R1 and R2. R2 contains the diferric-tyrosyl radical cofactor essential for the nucleotide reduction process. The in vitro mechanism of assembly of this cluster starting with apo R2 or with a diferrous form of R2 has been examined by time-resolved physical biochemical methods. An intermediate, Fe3+/Fe4+ cluster (intermediate X), has been identified that is thought to be directly involved in the oxidation of Y122 to the tyrosyl radical (*Y122). An R2 mutant in which phenylalanine has replaced Y122 has been used to accumulate intermediate X at sufficient levels that it can be studied using a variety of spectroscopic methods. The details of the reconstitution of the apo and diferrous forms of Y122F R2 have been examined by stopped-flow UV/vis spectroscopy and by rapid freeze quench electron paramagnetic resonance, and Mössbauer spectroscopies. In addition the structure of this mutant, crystallized at pH 7.6 in the absence of mercury, at 2.46 A resolution has been determined. These studies suggest that Y122F R2 is an appropriate model for the examination of intermediate X in the assembly process. Studies with two mutants, Y356F and double mutant Y356F and Y122F R2, are interpreted in terms of the possible role of Y356 in the putative electron transfer reaction between the R1 and R2 subunits of this RNR.

PubMed: 9558317
DOI: 10.1021/bi9728811

実験手法

X-RAY DIFFRACTION (2.46 Å)