2AUB

Lysozyme structure derived from thin-film-based crystals

2AUB の概要

• エントリーDOI: 10.2210/pdb2aub/pdb
• 分子名称: Lysozyme C (2 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14331.16

構造登録者

Pechkova, E.,Sivozhelezov, V.,Tropiano, G.,Fiordoro, S.,Nicolini, C. (登録日: 2005-08-27, 公開日: 2005-12-06, 最終更新日: 2024-11-13)

主引用文献

Pechkova, E.,Sivozhelezov, V.,Tropiano, G.,Fiordoro, S.,Nicolini, C.
Comparison of lysozyme structures derived from thin-film-based and classical crystals.
Acta Crystallogr.,Sect.D, 61:803-808, 2005

PubMed Abstract: The present report is dedicated to a systematic comparison of crystal structures produced by the nanobiofilm template method and by the classical hanging-drop vapour-diffusion method. Crystals grown by the innovative nanostructured template method appear indeed radiation-resistant even in the presence of a third-generation highly focused beam at the European Synchrotron Radiation Facility. The implications of this finding for protein crystallography are discussed here in terms of water redistribution and of the detailed atomic resolution comparative studies of the two crystal structures with or without nanobiofilm template, as emerging also from circular-dichroism and thermal denaturation studies.

PubMed: 15930644
DOI: 10.1107/S0907444905006578

実験手法

X-RAY DIFFRACTION (1.7 Å)