2ASB

Structure of a Mycobacterium tuberculosis NusA-RNA complex

2ASB の概要

• エントリーDOI: 10.2210/pdb2asb/pdb
• 関連するPDBエントリー: 2ATW
• 分子名称: ribosomal RNA (5'- GAACUCAAUAG -3'), Transcription elongation protein nusA (3 entities in total)
• 機能のキーワード: protein-rna complex, transcription-rna complex, transcription/rna
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30525.82

構造登録者

Beuth, B.,Pennell, S.,Arnvig, K.B.,Martin, S.R.,Taylor, I.A. (登録日: 2005-08-23, 公開日: 2005-10-11, 最終更新日: 2023-08-23)

主引用文献

Beuth, B.,Pennell, S.,Arnvig, K.B.,Martin, S.R.,Taylor, I.A.
Structure of a Mycobacterium tuberculosis NusA-RNA complex.
Embo J., 24:3576-3587, 2005

PubMed Abstract: NusA is a key regulator of bacterial transcriptional elongation, pausing, termination and antitermination, yet relatively little is known about the molecular basis of its activity in these fundamental processes. In Mycobacterium tuberculosis, NusA has been shown to bind with high affinity and specificity to BoxB-BoxA-BoxC antitermination sequences within the leader region of the single ribosomal RNA (rRNA) operon. We have determined high-resolution X-ray structures of a complex of NusA with two short oligo-ribonucleotides derived from the BoxC stem-loop motif and have characterised the interaction of NusA with a variety of RNAs derived from the antitermination region. These structures reveal the RNA bound in an extended conformation to a large interacting surface on both KH domains. Combining structural data with observed spectral and calorimetric changes, we now show that NusA binding destabilises secondary structure within rRNA antitermination sequences and propose a model where NusA functions as a chaperone for nascently forming RNA structures.

PubMed: 16193062
DOI: 10.1038/sj.emboj.7600829

実験手法

X-RAY DIFFRACTION (1.5 Å)