2ARX

Pterocarpus angolensis seed lectin in complex with the decasaccharide NA2F

2ARX の概要

• エントリーDOI: 10.2210/pdb2arx/pdb
• 関連するPDBエントリー: 2AR6 2ARB 2ARE
• 関連するBIRD辞書のPRD_ID: PRD_900112
• 分子名称: lectin, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: lectin, carbohydrate, legume lectin, sugar binding protein
• 由来する生物種: Pterocarpus angolensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57180.35

構造登録者

Buts, L.,Garcia-Pino, A.,Imberty, A.,Amiot, N.,Boons, G.-J.,Lah, J.,Versees, W.,Wyns, L.,Loris, R. (登録日: 2005-08-22, 公開日: 2006-08-01, 最終更新日: 2024-10-09)

主引用文献

Buts, L.,Garcia-Pino, A.,Imberty, A.,Amiot, N.,Boons, G.J.,Beeckmans, S.,Versees, W.,Wyns, L.,Loris, R.
Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin.
Febs J., 273:2407-2420, 2006

PubMed Abstract: The crystal structure of Pterocarpus angolensis lectin is determined in its ligand-free state, in complex with the fucosylated biantennary complex type decasaccharide NA2F, and in complex with a series of smaller oligosaccharide constituents of NA2F. These results together with thermodynamic binding data indicate that the complete oligosaccharide binding site of the lectin consists of five subsites allowing the specific recognition of the pentasaccharide GlcNAc beta(1-2)Man alpha(1-3)[GlcNAc beta(1-2)Man alpha(1-6)]Man. The mannose on the 1-6 arm occupies the monosaccharide binding site while the GlcNAc residue on this arm occupies a subsite that is almost identical to that of concanavalin A (con A). The core mannose and the GlcNAc beta(1-2)Man moiety on the 1-3 arm on the other hand occupy a series of subsites distinct from those of con A.

PubMed: 16704415
DOI: 10.1111/j.1742-4658.2006.05248.x

実験手法

X-RAY DIFFRACTION (2 Å)