2ARG

FORMATION OF AN AMINO ACID BINDING POCKET THROUGH ADAPTIVE ZIPPERING-UP OF A LARGE DNA HAIRPIN LOOP, NMR, 9 STRUCTURES

Summary for 2ARG

• Entry DOI: 10.2210/pdb2arg/pdb
• Descriptor: DNA APTAMER [5'-D (*TP*GP*AP*CP*CP*AP*GP*GP*GP*CP*AP*AP*AP*CP*GP*GP*TP*AP* GP*GP*TP*GP*AP*GP*TP*GP*GP*TP*CP*A)-3'], ARGININEAMIDE (2 entities in total)
• Functional Keywords: adaptive dna structural transitions, l-argininamide binding pocket, molecular recognition of an amino acid, minor groove recognition, base encapsulation within minor groove, dna aptamer, deoxyribonucleic acid, dna
• Total number of polymer chains: 1
• Total formula weight: 9552.26

Authors

Lin, C.H.,Wang, W.,Jones, R.A.,Patel, D.J. (deposition date: 1998-08-19, release date: 1999-03-23, Last modification date: 2024-05-22)

Primary citation

Lin, C.H.,Wang, W.,Jones, R.A.,Patel, D.J.
Formation of an amino-acid-binding pocket through adaptive zippering-up of a large DNA hairpin loop.
Chem.Biol., 5:555-572, 1998

PubMed Abstract: In vitro selection has identified DNA aptamers that target cofactors, amino acids, peptides and proteins. Structure determination of such ligand-DNA aptamer complexes should elucidate the details of adaptive DNA structural transitions, binding-pocket architectures and ligand recognition. We have determined the solution structure of the complex of a DNA aptamer containing a guanine-rich 18-residue hairpin loop that binds L-argininamide with approximately 100 microM affinity.

PubMed: 9818148
DOI: 10.1016/S1074-5521(98)90114-4

Experimental method

SOLUTION NMR