2AQY

(3+1) assembly of three human telomeric DNA repeats into an asymmetrical dimeric G-quadruplex

2AQY の概要

• エントリーDOI: 10.2210/pdb2aqy/pdb
• 分子名称: 5'-D(*GP*(OIP)P*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*T)-3', 5'-D(*TP*AP*GP*GP*GP*(DU))-3' (2 entities in total)
• 機能のキーワード: (3+1) g-quadruplex assembly, asymmetric dimeric g-quadruplex, telomeric dna., dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6900.49

構造登録者

Zhang, N.,Phan, A.T.,Patel, D.J. (登録日: 2005-08-18, 公開日: 2006-03-07, 最終更新日: 2024-05-22)

主引用文献

Zhang, N.,Phan, A.T.,Patel, D.J.
(3 + 1) Assembly of three human telomeric repeats into an asymmetric dimeric G-quadruplex
J.Am.Chem.Soc., 127:17277-17285, 2005

PubMed Abstract: We present an NMR study on the structure of a DNA fragment of the human telomere containing three guanine-tracts, d(GGGTTAGGGTTAGGGT). This sequence forms in Na(+) solution a unique asymmetric dimeric quadruplex, in which the G-tetrad core involves all three G-tracts of one strand and only the last 3'-end G-tract of the other strand. We show that a three-repeat human telomeric sequence can also associate with a single-repeat human telomeric sequence into a structure with the same topology that we name (3 + 1) quadruplex assembly. In this G-quadruplex assembly, there are one syn.syn.syn.anti and two anti.anti.anti.syn G-tetrads, two edgewise loops, three G-tracts oriented in one direction and the fourth oriented in the opposite direction. We discuss the possible implications of the new folding topology for understanding the structure of telomeric DNA, including t-loop formation, and for targeting G-quadruplexes in the telomeres.

PubMed: 16332077
DOI: 10.1021/ja0543090

実験手法

SOLUTION NMR